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Updated: Sep 18, 2026

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
Published on: April 28, 2014
[Simultaneous electrochemical determinations of total co2 and chlorides in biological fluids (author's transl)]
Abstract:
We thought it was interesting to study the results provided by an instrument intended for chlorides and total CO2 plasmatic determinations using specific electrodes. The main qualities observed are the following: -Chloride linearity 10 up to 300 mmol/I. -Total CO2 linearity 5 up to 50 mmol/I. The within run precision for chloride is 0,74% CV and for total CO2 is 1,56% CV. The between day precision is excellent: CI--0,95% CV. Total CO2 1,54% CV. --No contamination at all. --Throughput: 55 samples per hour. --Good correlation with the routine methods used in our laboratory CI--r = 0,90. Total CO2 r = 0,98. --No interferences at all by compounds such as hemoglobulin, bilirubin. --Possible measurement with urine, CSF, gastric fluid, ascites and pleural punctures and sweat. This instrument seems well adapted for pediatric, resuscitation and "stat" setting in a clinical laboratory.
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