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Fluorescence and Phosphorescence: Instrumentation

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

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Integrated instrumental analysis teaching platform with smartphone-operated fluorometer.

Lucas B Ayres¹, Fernando S Lopes, Carlos D Garcia

¹Department of Chemistry, Clemson University, Clemson, South Carolina 29634, USA.

Analytical Methods : Advancing Methods and Applications

|August 4, 2020

Summary

This study introduces an Integrated Teaching Tool (ITT) for analytical chemistry education, featuring a low-cost, 3D-printed fluorometer controlled by a smartphone app. The tool enhances remote data acquisition and student-instructor interaction for improved learning outcomes.

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Area of Science:

Analytical Chemistry
Educational Technology
Instrumental Analysis

Background:

Traditional analytical chemistry labs face challenges in accessibility and cost.
There is a need for innovative tools to enhance student engagement and practical learning.
Integrating portable instrumentation with mobile technology offers a novel educational approach.

Purpose of the Study:

To develop and present an Integrated Teaching Tool (ITT) for analytical chemistry.
To demonstrate the utility of a low-cost, 3D-printed fluorometer within an educational framework.
To create a mobile application for remote instrument control, data acquisition, and student assessment.

Main Methods:

Design and fabrication of a wireless, portable fluorometer using 3D printing and a Teensy 3.1 microcontroller.
Development of an Android application for Bluetooth-based remote control, data plotting, and result transmission.
Integration of educational content on fluorescence and instrument details within the application.
Implementation of a notification system for instructor-student communication and evaluation.

Main Results:

Successful development of a functional, low-cost, 3D-printed fluorometer.
Demonstration of remote data acquisition and analysis via a smartphone application.
Creation of an integrated platform combining hardware, software, and educational resources.
Facilitation of enhanced student-instructor interaction and assessment capabilities.

Conclusions:

The Integrated Teaching Tool (ITT) effectively supports analytical chemistry education by providing accessible, portable instrumentation.
The 3D-printed fluorometer and accompanying mobile application offer a cost-effective solution for practical learning.
The platform enhances remote learning capabilities and streamlines student evaluation, promoting a more interactive educational experience.