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Effects of microRNA-21 targeting PITX2 on proliferation and apoptosis of pituitary tumor cells

M Cui1, M Zhang, H-F Liu

  • 1Department of Neurology, The 5th Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

European Review for Medical and Pharmacological Sciences
|August 4, 2020
PubMed

Insights

This study on microRNA-21 targeting PITX2 in pituitary tumor cells has been withdrawn due to suspected research misconduct. The publisher retracted the article after authors failed to verify data originality.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Oncology

Background:

  • Pituitary tumors are complex neoplasms.
  • MicroRNAs play a role in cellular processes.
  • PITX2 is implicated in pituitary development.

Purpose of the Study:

  • To investigate the role of microRNA-21 (miR-21) in regulating PITX2 expression.
  • To determine the effect of targeting PITX2 with miR-21 on pituitary tumor cell proliferation and apoptosis.

Main Methods:

  • In vitro cell culture models of pituitary tumors.
  • Quantitative real-time PCR to measure gene and microRNA expression.
  • Cell proliferation assays (e.g., MTT assay).
  • Apoptosis assays (e.g., flow cytometry, TUNEL assay).

Main Results:

  • microRNA-21 was found to target PITX2.
  • Targeting PITX2 with miR-21 affected pituitary tumor cell proliferation.
  • The study indicated an impact on apoptosis in these cells.

Conclusions:

  • microRNA-21 plays a role in regulating PITX2 in pituitary tumor cells.
  • Modulation of PITX2 by miR-21 influences tumor cell behavior.
  • Further research is warranted to explore therapeutic strategies.

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