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Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Related Experiment Video

Updated: Dec 13, 2025

Two Different Real-Time Place Preference Paradigms Using Optogenetics within the Ventral Tegmental Area of the Mouse

Two Different Real-Time Place Preference Paradigms Using Optogenetics within the Ventral Tegmental Area of the Mouse

Published on: February 12, 2020

CreLite: An optogenetically controlled Cre/loxP system using red light.

Shuo-Ting Yen¹, Kenneth A Trimmer^1,2, Nader Aboul-Fettouh¹

¹Department of Genetics, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|August 4, 2020

Summary

This summary is machine-generated.

We developed CreLite, a novel optogenetic system for precise gene manipulation in zebrafish. This red-light-inducible system enables temporal and spatial control of gene expression, advancing functional analysis in developmental biology.

Keywords:

Cre/loxP gene expression optogenetics zebrafish

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Last Updated: Dec 13, 2025

Two Different Real-Time Place Preference Paradigms Using Optogenetics within the Ventral Tegmental Area of the Mouse

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Published on: February 12, 2020

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Published on: July 6, 2021

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

Area of Science:

Developmental Biology
Genetics
Optogenetics

Background:

Precise control of gene expression is crucial for understanding biological systems.
The Cre-loxP system is widely used but relies on specific regulatory elements for control.
Existing methods lack sufficient temporal and spatial specificity in certain applications.

Purpose of the Study:

To develop a novel optogenetically controlled Cre recombinase system.
To achieve precise temporal and spatial control of gene expression using red light.
To validate the CreLite system in developing zebrafish embryos.

Main Methods:

Engineered Cre recombinase by splitting it into two inactive components fused with red light-inducible proteins (PhyB and PIF6).
Utilized the cofactor phycocyanobilin (PCB) to reconstitute Cre activity upon red light exposure.
Injected CreLite mRNAs and PCB into zebrafish embryos carrying a Cre-dependent reporter system.

Main Results:

Demonstrated successful Cre activity restoration and gene manipulation in zebrafish embryos upon red light illumination.
Achieved multispectral cell labeling in various tissues, indicating successful spatiotemporal control.
Showcased the ability to target gene manipulation to whole embryos or specific cell groups.

Conclusions:

CreLite provides a powerful tool for temporal and spatial gene manipulation in zebrafish.
The system allows for precise control of gene expression at different developmental stages.
CreLite holds potential for applications in cell culture, organ culture, and other animal models.