Related Experiment Video
Updated: Dec 13, 2025
10:40
Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
Published on: December 21, 2019
26.3K
Host antiviral protein IFITM2 restricts pseudorabies virus replication
Jingying Xie1, Yingjie Bi2, Shujuan Xu2
1College of Veterinary Medicine, Gansu Agricultural University, No.1 Yingmencun, Lanzhou, 730070, China; Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China.
Virus Research
|August 4, 2020
Summary
Interferon-induced transmembrane (IFITM) proteins are crucial for controlling Pseudorabies virus (PRV) infection in pigs. IFITM proteins block PRV entry by interacting with the cholesterol pathway.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Pseudorabies virus (PRV) causes significant economic losses in the global pig industry.
- Type I interferons (IFNs) are key to innate immunity, inducing interferon-stimulated genes (ISGs).
- IFN-induced transmembrane (IFITM) proteins are ISGs known for broad-spectrum antiviral activity against various viruses.
Purpose of the Study:
- To investigate the role of IFITM proteins in Pseudorabies virus (PRV) infection.
- To elucidate the mechanism by which IFITMs restrict PRV.
- To understand the host-pathogen interactions during PRV infection.
Main Methods:
- Assessing the impact of IFITM proteins on PRV infection.
- Analyzing PRV cell binding and entry processes.
- Investigating the involvement of the cholesterol pathway in IFITM-mediated antiviral activity.
Main Results:
- IFITM proteins are essential for controlling PRV infection.
- IFITM proteins inhibit PRV cell binding and viral entry.
- IFITM2's inhibition of PRV entry is dependent on the cholesterol pathway.
Conclusions:
- IFITM proteins play a critical role in restricting PRV infection.
- The anti-PRV mechanism of IFITMs involves interference with viral entry, potentially via cholesterol pathway modulation.
- Cholesterol's importance in virus infection is further highlighted by these findings.
Related Concept Videos
02:28Leaky Scanning
5.5K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.5K
01:29Viruses with RNA Genomes
540
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
540
01:29Subviral Agents
362
Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
362
02:15Experimental RNAi
7.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.1K
01:10Retrovirus Life Cycles
48.9K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
48.9K