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Throughput-Speed Product Augmentation for Scanning Fiber-Optic Two-Photon Endomicroscopy
IEEE Transactions on Medical Imaging
|August 4, 2020
Summary
This study introduces a novel fiber-optic scanning endomicroscope design that enhances imaging throughput and speed. The new strategy significantly improves imaging area or frame rate for advanced in vivo microscopy applications.
Area of Science:
- Biomedical Engineering
- Optical Imaging
- Microscopy
Background:
- Compactness is a key feature of fiber-optic two-photon endomicroscopes.
- Increasing scanning area and pixels (throughput) typically reduces scanning speed.
- Existing designs face trade-offs between imaging area and speed.
Purpose of the Study:
- To introduce a new design strategy for fiber-optic scanning endomicroscopes.
- To enhance both imaging area (throughput) and scanning speed.
- To overcome limitations of traditional designs.
Main Methods:
- Distributed the numerical aperture (NA) or beam focusing power over two stages.
- Engineered a mode-field focuser at the tip of a double-clad fiber (DCF) cantilever.
- Utilized a low-magnification micro objective for final beam focusing.
Main Results:
- Achieved an approximately 9-fold increase in imaging area (throughput) or a 3-fold improvement in imaging frame rate.
- Demonstrated high-resolution in vivo imaging of mouse kidney over a large field of view without contrast agents.
- Showcased real-time neural imaging of dendritic calcium dynamics in vivo with sub-second temporal resolution.
Conclusions:
- The cascaded NA amplification strategy enhances throughput-speed product in fiber-optic scanners.
- This design is adaptable to various compact linear or nonlinear endomicroscopes.
- The approach offers significant improvements for in vivo microscopy applications.
