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Published on: April 28, 2016
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Dynamic of TLQP-peptides upon fasting
Barbara Noli1, Carla Brancia1, Giulia Corda1
1NEF-Laboratory, Department of Biomedical Sciences, University of Cagliari, 09042, Monserrato, CA, Italy.
Tissue & Cell
|August 5, 2020
Summary
Fasting down-regulates stomach, pancreas, adrenal gland, and adipose tissue TLQP peptides (TLQPp), increasing blood levels. This suggests TLQPp are secreted into circulation during fasting to counteract hypoglycemia.
Area of Science:
- Endocrinology
- Metabolism
- Neuroscience
Background:
- VGF-derived TLQP peptides (TLQPp) are potential drug targets for obesity, regulating energy expenditure and food intake.
- Physiological changes of TLQPp in response to fasting in key organs remain unclear.
Purpose of the Study:
- To investigate the physiological changes of TLQPp in rats during fasting.
- To explore the relationship between TLQPp, VGF precursor, and TLQP-21 receptors under fasting conditions.
Main Methods:
- Rats were subjected to different feeding regimens: ad libitum, restricted feeding, 48-hour fasting, and 48-hour fasting followed by 1-hour refeeding.
- Immunosorbent assay (ELISA) was used to measure TLQPp and VGF precursor levels in plasma and various tissues (pancreas, adrenal gland, stomach, adipose tissue).
- Immunohistochemistry was employed to detect TLQP-21 receptors.
Main Results:
- Fasting led to significant down-regulation of TLQPp in the stomach (45%), pancreas (47%), adrenal gland (51%), and white adipose tissue (WAT) (45.2%).
- Concurrently, blood TLQPp levels increased by 36.6% during fasting compared to the ad libitum group.
- VGF precursor levels remained unchanged in fasting-affected organs, while TLQP-21 receptors showed co-localization with TLQPp in ad libitum rats, suggesting autocrine signaling.
Conclusions:
- Fasting induces a decrease in TLQPp within specific organs, accompanied by an increase in circulating TLQPp.
- These findings suggest that TLQPp are likely produced and rapidly secreted into the bloodstream during fasting to help manage hypoglycemia.
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