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Updated: Dec 13, 2025

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Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
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Local auxin biosynthesis is required for root regeneration after wounding
Rotem Matosevich1, Itay Cohen1, Naama Gil-Yarom1
1Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
Nature Plants
|August 5, 2020
Summary
Plant root regeneration depends on new auxin sources forming near the injury. Continuous auxin synthesis, not transport, is crucial for stem cell niche repair and meristem regrowth.
Area of Science:
- Plant Biology
- Developmental Biology
- Molecular Biology
Background:
- Plant root meristem stem-cell niche regeneration involves remnant cell recruitment.
- Auxin accumulation at the injury site initiates root regeneration, but its source remains unclear.
Purpose of the Study:
- To identify the source of auxin accumulation during root regeneration.
- To investigate the role of auxin biosynthesis and transport in meristem regeneration.
- To understand the factors controlling regeneration competence.
Main Methods:
- Investigated auxin accumulation using live imaging and chemical treatments.
- Assessed the role of auxin biosynthesis and PIN-mediated transport.
- Utilized genetic mutants (erf115) and tissue-specific regeneration assays.
Main Results:
- Auxin accumulation originates from newly specified, localized auxin biosynthetic sources near the cut site.
- Continuous activity of these local auxin sources is essential for regeneration.
- PIN-mediated auxin transport is dispensable for auxin accumulation and tip regeneration.
- ERF115 activity and specific tissue zones are critical for activating local auxin sources.
Conclusions:
- Root regeneration competence is linked to the ability to establish precise, local auxin biosynthesis.
- Restoring local auxin supply can rescue regeneration capacity in recalcitrant tissues.
- Regeneration relies on temporally patterned specification of new auxin sources.
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