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Cross-reactive antigens and HLA matching.
1Department of Pathology, University of Florida College of Medicine, Gainesville 32610.
Transplantation Proceedings
|February 1, 1987
Summary
Broadly reactive sera can show anti-HLA-A2 and anti-B17 reactivity, even without matching antigens. This suggests predictions of negative cross-matches based on antigen cross-reactivity are often inaccurate.
Area of Science:
- Immunology
- Transplantation immunology
Background:
- Broadly reactive antibodies (BRAs) pose challenges in solid organ transplantation.
- Accurate prediction of antibody reactivity is crucial for successful cross-matching.
Purpose of the Study:
- To investigate the reactivity of broadly reactive sera against HLA-A2 and HLA-B17 specificities.
- To assess the reliability of antigen cross-reactivity for predicting negative cross-matches.
Main Methods:
- Studied 87 broadly reactive sera.
- Assessed anti-HLA-A2 and anti-B17 reactivity using inhibition of monoclonal antibody binding.
Main Results:
- Broadly reactive sera frequently exhibited anti-HLA-A2 and anti-B17 reactivity.
- This reactivity was observed in patients with and without antigens cross-reactive with A2 or B17.
- A high proportion of cases showed inaccurate predictions of negative cross-matches.
Conclusions:
- Reactivity against public epitopes or antigen cross-reactivity is not a reliable predictor of negative cross-matches.
- Clinical decisions based solely on predicted negative cross-matches may be flawed.