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Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Circulating microparticle concentrations across acute and chronic cardiovascular disease conditions
Rian Q Landers-Ramos1,2,3, Odessa A Addison2,3, Brock Beamer2,3
1Department of Kinesiology, Towson University, Towson, MD, USA.
Insights
Circulating microparticles (MPs) like CD31+/CD42b- may serve as biomarkers for cardiovascular disease (CVD). Lower CD31+/CD42b- MP levels were observed in patients with coronary artery disease (CAD) and non-ST elevation myocardial infarction (NSTEMI).
Area of Science:
- Cardiovascular Science
- Biomarker Discovery
- Hematology
Background:
- Circulating microparticles (MPs) are implicated in cardiovascular disease (CVD) pathologies.
- Specific MP subtypes may hold diagnostic or prognostic value in CVD.
- Understanding MP concentrations in relation to CVD is crucial for clinical applications.
Purpose of the Study:
- To quantify plasma concentrations of CD31+/CD42b-, CD62E+, and CD34+ MPs.
- To compare MP levels between healthy individuals and patients with coronary artery disease (CAD) or non-ST elevation myocardial infarction (NSTEMI).
- To assess relationships between MP subtypes and clinical parameters.
Main Methods:
- Plasma samples were collected from healthy older men, CAD patients, and NSTEMI patients.
- Flow cytometry was used to isolate and quantify CD31+/CD42b-, CD62E+, and CD34+ MPs.
- Statistical analyses examined correlations between MP concentrations and clinical variables.
Main Results:
- CD31+/CD42b- MP concentrations were significantly lower in CAD and NSTEMI groups compared to healthy controls.
- No significant differences in CD62E+ or CD34+ MP levels were found between the groups.
- CD62E+ MP concentrations showed a positive correlation with triglycerides and an inverse correlation with systolic blood pressure (SBP).
Conclusions:
- CD31+/CD42b- MPs show potential as a biomarker for cardiovascular disease.
- MP concentrations are influenced by complex interactions including comorbidities and medications.
- Further research is needed to elucidate the role of various MP subtypes in CVD.
Abstract:
Concentrations of different circulating microparticles (MPs) may have clinical and physiological relevance to cardiovascular disease pathologies.
Purpose:
To quantify plasma concentrations of CD31+/CD42b-, CD62E+, and CD34+ MPs across healthy individuals and those with coronary artery disease (CAD) or acute cardiovascular events (non-ST elevation myocardial infarction (NSTEMI)). Fasted blood was obtained from CAD patients (n = 10), NSTEMI patients (n = 13), and healthy older men (n = 15) 60-75 years old.
Methods:
CD31+/CD42b-, CD62E+, and CD34+ MPs were isolated from plasma and quantified using flow cytometry. Relationships between MP subtypes, fasting blood lipids, blood glucose, blood pressure, body mass index, and total number of medications were assessed.
Results:
Concentrations of CD31+/CD42b- MPs were significantly lower in CAD and NSTEMI subjects compared with healthy individuals (p = .02 and .003, respectively). No differences between groups were found for CD62E+ or CD34+ MPs (p > .05 for both). Surprisingly, among all variables assessed, only CD62E+ MP concentrations were positively correlated with triglyceride levels (p = .012) and inversely correlated with SBP (p = .03).
Conclusions:
Our findings provide support for the use of different MP subtypes, specifically CD31+/CD42b- MPs, as a potential biomarker of cardiovascular disease. Importantly, results from this study should be looked at in adjunct to previous MP work in CVD conditions as a way of highlighting the complex interactions of variables such as comorbid conditions and medications on MP concentrations.
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