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Targeting CD47 Inhibits Tumor Development and Increases Phagocytosis in Oral Squamous Cell Carcinoma
Xiao-Jing Ye1, Jian-Guang Yang1, Ya-Qin Tan1
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
Background:
Our previous work demonstrated upregulated CD47 in Oral Squamous Cell Carcinoma (OSCC).
Objective:
In the present study, we aimed to investigate the effects of CD47 on tumor cell development and phagocytosis in OSCC and elucidate the underlying mechanisms.
Methods:
The proliferation, apoptosis, migration, and invasion of oral cancer cells were analyzed after knocking down the expression of CD47. The effects of CD47 on tumor development were also evaluated using a murine model of OSCC. The involvement of CD47 in the phagocytosis of oral cancer cells was identified.
Results:
Cell proliferation was suppressed by knocking down the expression of CD47 in human OSCC cell line Cal-27 cells but there was no change in the apoptosis rate. Moreover, impaired expression of CD47 inhibited the migration and invasion of Cal-27 cells. Furthermore, we found that nude mice injected with CD47 knockeddown Cal-27 cells displayed decreased tumor volumes at week 9 compared to xenograft transplantations of blank Cal-27 cells. In addition, in vitro phagocytosis of Cal-27 cells by macrophages was significantly enhanced after the knockdown of CD47, which positively correlated with compromised STAT3/JAK2 signaling.
Conclusion:
In summary, the knockdown of CD47 downregulated the development of OSCC and increased the phagocytosis of Cal-27 cells, indicating that CD47 might be a promising therapeutic target.
Insights
Downregulating CD47 in Oral Squamous Cell Carcinoma (OSCC) suppressed tumor growth and enhanced macrophage phagocytosis. This suggests CD47 is a potential therapeutic target for OSCC treatment.
Area of Science:
- Oncology
- Immunology
- Cancer Biology
Background:
- Previous research identified upregulated CD47 in Oral Squamous Cell Carcinoma (OSCC).
- CD47 plays a role in immune evasion and tumor progression.
Purpose of the Study:
- To investigate the impact of CD47 on OSCC cell development and phagocytosis.
- To elucidate the mechanisms by which CD47 influences OSCC.
Main Methods:
- CD47 expression was knocked down in human OSCC cell line Cal-27.
- Cell proliferation, apoptosis, migration, and invasion were analyzed.
- Tumor development was assessed in a murine OSCC model.
- In vitro phagocytosis assays were performed.
Main Results:
- Knockdown of CD47 suppressed Cal-27 cell proliferation, migration, and invasion.
- Tumor volumes were reduced in mice with CD47-knockdown OSCC cells.
- Macrophage phagocytosis of Cal-27 cells was significantly enhanced upon CD47 knockdown.
- Enhanced phagocytosis correlated with compromised STAT3/JAK2 signaling.
Conclusions:
- CD47 knockdown downregulates OSCC development.
- Targeting CD47 increases phagocytosis of oral cancer cells.
- CD47 represents a promising therapeutic target for OSCC.
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