Related Experiment Video
Updated: Dec 13, 2025

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy
Published on: June 15, 2022
Chaotic markers in dynamic diffraction
Abstract:
In a dynamic far-field diffraction experiment, we calculate the largest Lyapunov exponent of a time series obtained from the optical fluctuations in a dynamic diffraction pattern. The time series is used to characterize the locomotory predictability of an oversampled microscopic species. We use a live nematode, Caenorhabditis elegans, as a model organism to demonstrate our method. The time series is derived from the intensity at one point in the diffraction pattern. This single time series displays chaotic markers in the locomotion of the Caenorhabditis elegans by reconstructing the multidimensional phase space. The average largest Lyapunov exponent (base e) associated with the dynamic diffraction of 10 adult wildtype (N2) Caenorhabditis elegans is 1.27±0.03s-1.
Related Concept Videos
Interference and Diffraction
X-ray Crystallography
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
X-ray Diffraction of Biological Samples
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

