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Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Experimental RNAi02:15

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
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Related Experiment Video

Updated: Dec 13, 2025

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
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Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

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Modulating Polymer-siRNA Binding Does Not Promote Polyplex-Mediated Silencing.

R Chauncey Splichal1, Joseph A Gredell1,2, Erin B Vogel3,4

  • 1Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan, USA.

Nucleic Acid Therapeutics
|August 5, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed novel cationic polymers for delivering small interfering RNAs (siRNAs). While these polymers showed strong binding and no toxicity, they failed to achieve protein silencing, highlighting challenges in siRNA delivery vehicle design.

Keywords:
cationic polymerspoly(propargyl glycolide)siRNA delivery

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Porous Silicon Microparticles for Delivery of siRNA Therapeutics
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Area of Science:

  • Biotechnology
  • Polymer Chemistry
  • Gene Therapy

Background:

  • Developing effective delivery vehicles for small interfering RNAs (siRNAs) is crucial for their clinical application.
  • Cationic polymers are a promising class of materials for siRNA delivery.

Purpose of the Study:

  • To synthesize and evaluate novel cationic poly(propargyl glycolide) backbone polymers for siRNA binding and delivery.
  • To investigate the relationship between polymer binding strength and siRNA delivery efficiency.

Main Methods:

  • Utilized alkyne-azide click chemistry for polymer synthesis.
  • Quantified polymer-siRNA binding strength across a wide range.
  • Assessed polymer cytotoxicity and siRNA delivery in cellular models.

Main Results:

  • Successfully synthesized cationic polymers with tunable siRNA binding strengths exceeding commercial agents.
  • Demonstrated effective siRNA delivery with no detectable cytotoxicity.
  • Observed siRNA accumulation comparable to commercial reagents but without targeted protein silencing.

Conclusions:

  • The synthesized polymers show potential as safe siRNA delivery vehicles with tunable binding.
  • Further optimization is needed to overcome the lack of gene silencing despite successful siRNA delivery.
  • Results provide insights into designing future siRNA delivery systems.