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SR7 - a dual-function antisense RNA from Bacillus subtilis.

Inam Ul Haq1, Peter Müller1, Sabine Brantl1

  • 1Friedrich-Schiller-Universität Jena, Matthias-Schleiden-Institut , AG Bakteriengenetik, Jena, Germany.

RNA Biology
|August 6, 2020
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Summary
This summary is machine-generated.

A novel dual-function RNA, SR7, in Bacillus subtilis encodes a protein (SR7P) that interacts with enolase, enhancing RNase Y activity and fine-tuning mRNA degradation. This discovery reveals a new layer of gene regulation under stress conditions.

Keywords:
bacillus subtilisDual-function antisense RNARNA degradationdegradosomeenolaseregulatory peptidernase Ysr7sr7p

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Area of Science:

  • Molecular Biology
  • Bacterial Genetics
  • RNA Biology

Background:

  • Bacillus subtilis possesses a regulatory RNA, SR7, previously identified as S1136, involved in stress response.
  • SR7 was known to regulate small ribosomal subunit levels under ethanol stress.

Purpose of the Study:

  • To investigate the dual functions of the SR7 RNA in Bacillus subtilis.
  • To characterize the protein encoded by SR7 and its role in cellular processes.

Main Methods:

  • Identification and characterization of the SR7 protein (SR7P) and its encoding mRNA.
  • Co-elution and Far-Western blotting to determine protein interactions.
  • In vitro degradation assays and Northern blot analysis to assess RNA degradation dynamics.

Main Results:

  • SR7 encodes a 39-amino acid protein, SR7P, translated from both SigB-dependent and independent transcripts.
  • SR7P interacts with the glycolytic enzyme enolase, a component of the Bacillus subtilis degradosome.
  • SR7P enhances the binding of RNase Y to enolase and increases the in vitro RNA degradation activity of enolase-bound RNase Y, fine-tuning rpsO mRNA half-life.

Conclusions:

  • SR7 is a dual-function molecule, acting as both an RNA regulator and a protein (SR7P) involved in mRNA degradation.
  • SR7P modulates the activity of the degradosome by interacting with enolase and influencing RNase Y function.
  • This study reveals a novel mechanism for post-transcriptional gene regulation in bacteria, particularly under stress conditions.