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lncRNA TMPO-AS1 Exerts Oncogenic Roles in HCC Through Regulating miR-320a/SERBP1 Axis

Zhenchang Wang1, DanDan Huang2, Jingjing Huang3

  • 1Department of Spleen and Stomach Liver Disease, International Zhuang Hospital District of Guangxi University of Chinese Medicine, Nanning, Guangxi, People's Republic of China.

Oncotargets and Therapy
|August 6, 2020
PubMed
Abstract

Insights

Long non-coding RNA TMPO-AS1 promotes hepatocellular carcinoma (HCC) aggressiveness by sponging miR-320a, increasing SERBP1 levels, and driving tumor growth. This study clarifies TMPO-AS1

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNA (lncRNA) TMPO-AS1 is implicated in various cancers' aggressiveness.
  • The specific role of TMPO-AS1 in hepatocellular carcinoma (HCC) remains unclear.

Purpose of the Study:

  • To investigate the function and mechanism of TMPO-AS1 in HCC.
  • To determine the relationship between TMPO-AS1, miR-320a, and SERBP1 in HCC.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for TMPO-AS1 and miR-320a expression.
  • Cell viability, migration, and invasion assays (colony formation, wound healing, Transwell).
  • Bioinformatics, luciferase reporter, and FISH assays for molecular interactions.
  • Western blot for SERBP1 expression and in vivo tumor growth models.

Main Results:

  • TMPO-AS1 is overexpressed in HCC tissues and cell lines, correlating with advanced stage and poor prognosis.
  • TMPO-AS1 depletion inhibits HCC cell viability, migration, and invasion; upregulation has opposite effects.
  • TMPO-AS1 acts as a sponge for miR-320a in the cytoplasm, increasing SERBP1 levels and promoting tumor growth in vivo.

Conclusions:

  • TMPO-AS1 promotes aggressive behaviors in hepatocellular carcinoma.
  • TMPO-AS1 functions by regulating the miR-320a/SERBP1 axis.
Keywords:
TMPO-AS1hepatocellular carcinomainvasionmiR-320amigration

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