Studies on a new antibiotic--Chuangxinmycin

A new antibiotic, designated Chuangxinmycin and produced by a new species of microorganism Actinoplanes tsinanensis n. sp., has been isolated in crystalline form. Assay methods, fermentation and isolation procedures for the antibiotic are described. UV-IR-, NMR- and mass spectra as well as specific rotation of the antibiotic are given. Its molecular formula is C12H11NO2S. Studies on its chemical structure and total synthesis have been completed. According to its chemical structure, Chuangxinmycin is a new type of compound of natural origin. It is active in vitro against a number of Gram-negative and Gram-positive bacteria, whereas in vivo it is active in mice against Escherichia coli and Shigella dysenteriae infections. It shows a low toxicity in mice, LD50 being 600 mg/kg (iv) and 1,770 mg/kg (p. o.) respectively. Preliminary clinical results show that Chuangxinmycin is effective in the treatment of septicaemia, urinary and biliary infections caused by Escherichia coli.