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Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization
Published on: January 7, 2016
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Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle
Sara Darbandi1, Mahsa Darbandi1, Ashok Agarwal2
1Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
International Journal of Reproductive Biomedicine
|August 6, 2020
Summary
Asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT) significantly improves the developmental potential of aged human oocytes. This three-parent assisted reproductive technique offers a promising solution for enhancing oocyte competence in fertility treatments.
Area of Science:
- Reproductive biology
- Assisted reproductive technologies
- Oocyte maturation
Background:
- Three-parent assisted reproductive techniques may enhance oocyte competence.
- Human oocyte aging presents challenges in assisted reproduction.
Purpose of the Study:
- To investigate the efficacy of four assisted reproductive techniques for maturing aged human non-surrounded nucleolus germinal vesicle-stage (NSN-GV) oocytes.
- To compare germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT), and asynchronous ooplasmic transfer using waste MII oocyte (waOT).
Main Methods:
- NSN-GV oocytes were divided into four groups: GVT, sOT, caOT, waOT, and a control group.
- Key parameters assessed included fusion rates, progression to metaphase I (MI) and metaphase II (MII), fertilization, and cleavage rates to 2-, 4-, and 8-cell stages.
Main Results:
- Germinal vesicle transfer (GVT) showed no fusion. Synchronous ooplasmic transfer (sOT) and asynchronous ooplasmic transfer (waOT) demonstrated varying degrees of success.
- Asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT) resulted in the highest survival rate (p < 0.05) compared to sOT, waOT, and the control group.
- caOT group showed superior oocyte maturation, fertilization, and cleavage rates compared to other methods.
Conclusions:
- Asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT) is a beneficial method for improving the developmental capacity of aged human NSN-GV oocytes.
- caOT demonstrates superior outcomes compared to sOT, waOT, and GVT, offering a potential advancement in assisted reproductive technologies for aged oocytes.
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