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Updated: Dec 12, 2025

Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
Molecular detection of Mycobacterium tuberculosis in poor-quality cough specimens
Tondani A Mboneni1, Owen O Eales2, Ntsoaki L Mosina3
1Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.
Abstract:
Clinical specimens unfit for laboratory processing represent missed opportunities for diagnosing tuberculosis. Poor-quality cough specimens (n=61) from presumptive tuberculosis cases were cultured and GeneXpert MTB/RIF (Xpert) successfully performed on samples transferred by flocked swab into PrimeStore molecular transport medium (PS-MTM). Mycobacterium tuberculosis was grown in culture from 13 (21.3 %) and Xpert reported 15 (24.2 %) positive, of which 10 concordant. RT-PCR of PS-MTM samples showed enhanced sensitivity; three positives were missed by Xpert, five by culture and three more detected for a total of 21 positives (34.4 %).

