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Enzyme-Linked Immunosorbent Assay01:33

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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Related Experiment Video

Updated: Dec 12, 2025

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
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Highly sensitive optoelectrical biosensor for multiplex allergy diagnosis.

Salvador Mas1, Ahmed A Badran1, María-José Juárez1

  • 1Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Spain.

Biosensors & Bioelectronics
|August 7, 2020
PubMed
Summary
This summary is machine-generated.

We developed a cost-effective optoelectrical biosensor for simultaneous detection of total and allergen-specific immunoglobulin E (IgE) and immunoglobulin G (IgG) antibodies. This novel system shows high sensitivity and specificity for allergy diagnosis.

Keywords:
AllergensComponent-based diagnosticsIgEIgGIn vitro biosensingMultiplex

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Area of Science:

  • Biomedical Engineering
  • Immunology
  • Analytical Chemistry

Background:

  • Multiplexed biosensor systems are crucial for diagnosing diseases requiring multiple biomarker detection.
  • Hypersensitivity Immunoglobulin E (IgE) mediated syndromes involve allergen sensitization, making IgE assessment vital for allergy diagnosis.

Purpose of the Study:

  • To develop a reliable, flexible, and cost-effective optoelectrical biosensor for simultaneous determination of total and allergen-specific IgE and IgG antibodies.
  • To validate the biosensor's performance using human serum samples against a reference clinical assay.

Main Methods:

  • Construction of a multiplexed biosensor on a digital versatile disc (DVD) microarray format, immobilizing 12 allergen extracts/proteins.
  • Utilizing an immunogold-silver signal amplification method for antibody detection.
  • Validation using 127 human serum samples and comparison with a clinical immunofluorescence assay.

Main Results:

  • The biosensor achieved limits of detection of 0.26 IU/mL for IgE and 14 ng/mL for IgG.
  • Demonstrated high sensitivity (97.6%) and specificity (85.7%) in human serum samples.
  • Achieved an excellent Area Under the Curve (AUC) of 0.977, confirming strong correlation with the reference assay.

Conclusions:

  • The developed optoelectrical biosensor enables simultaneous, autonomous detection of IgE and IgG antibodies for allergy diagnosis.
  • Its cost-effectiveness and flexibility make it suitable for clinical and mobile laboratory settings, including large-scale allergy screening.
  • This technology offers a promising tool for defining individual sensitization profiles and improving allergy management.