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Related Concept Videos

Pinocytosis00:38

Pinocytosis

4.0K
Cells use energy-requiring bulk transport mechanisms to transfer large particles or large numbers of small particles into or out of the cell. The cells envelop the particles in spherical membranes called vesicles or vacuoles. Vesicles that transport material into the cell are built from the cell membrane. These vesicles encapsulate external molecules and transport them into the cell in a process called endocytosis.
Pinocytosis ("cellular drinking") is one of three main types of...
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Pinocytosis00:43

Pinocytosis

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Cells use energy-requiring bulk transport mechanisms to transfer large particles, or large amounts of small particles, into or out of the cell. The cells envelop the particles in spherical membranes called vesicles or vacuoles. Vesicles that transport material into the cell are built from the cell membrane. These vesicles encapsulate external molecules and transport them into the cell in a process called endocytosis.
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Endocytosis01:16

Endocytosis

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Eukaryotic cells acquire nutrients for growth and proliferation. Nutrients and other molecules that require degradation are internalized from the extracellular space by a process called endocytosis. The term ‘endocytosis' was first coined by Christian de Duve in 1963.
Endocytosis always begins with the plasma membrane enclosing an incoming molecule to form a transport vesicle which, in some cases, can be coated with a protein called ‘clathrin.' Endocytosed material is either...
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Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

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Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
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Phagocytosis00:41

Phagocytosis

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Cells pull particles inward and engulf them in spherical vesicles in an energy-requiring process called endocytosis. Phagocytosis ("cellular eating") is one of three major types of endocytosis. Cells use phagocytosis to take in large objects, such as other cells (or their debris), bacteria, and even viruses.
The objective of phagocytosis is often destruction. Cells use phagocytosis to eliminate unwelcome visitors, like pathogens (e.g., viruses and bacteria). Many immune system cells,...
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Phagocytosis00:41

Phagocytosis

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Cells pull particles inward and engulf them in spherical vesicles in an energy-requiring process called endocytosis. Phagocytosis (“cellular eating”) is one of three major types of endocytosis. Cells use phagocytosis to take in large objects—such as other cells (or their debris), bacteria, and even viruses.
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Related Experiment Video

Updated: Dec 12, 2025

Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
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Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

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Macropinocytosis in Different Cell Types: Similarities and Differences.

Xiao Peng Lin1, Justine D Mintern1, Paul A Gleeson1

  • 1The Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne 3010, Victoria, Australia.

Membranes
|August 7, 2020
PubMed
Summary

Macropinocytosis, a cellular process for internalizing large volumes, has diverse pathways and functions. This study compares macropinocytosis mechanisms across cell types to understand its physiological roles.

Keywords:
amiloridecancer cellsdendritic cellsendothelial cellsepithelial cellsmacrophagesmacropinocytosismacropinosomesmembrane rufflingmicroglianeurons

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Last Updated: Dec 12, 2025

Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
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High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry
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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Physiology

Background:

  • Macropinocytosis is a non-specific endocytic pathway involving the uptake of extracellular fluid and solutes via macropinosomes.
  • This process is crucial for physiological functions such as antigen presentation, nutrient sensing, and cell migration.
  • Recent research highlights the existence of multiple, distinct macropinocytosis pathways utilized by different cell types and triggered by various stimuli.

Purpose of the Study:

  • To compare macropinocytosis mechanisms across different primary and immortalized cell types.
  • To identify current knowledge gaps in understanding macropinocytosis regulation and function.
  • To discuss potential approaches for analyzing macropinocytosis function in vivo.

Main Methods:

  • Comparative analysis of macropinocytosis mechanisms.
  • Literature review to identify knowledge gaps.
  • Discussion of in vivo analysis techniques.

Main Results:

  • Identification of diverse macropinocytosis pathways across different cell types.
  • Highlighting of specific stimuli triggering distinct macropinocytosis pathways.
  • Recognition of the need for further research into the regulation and in vivo functions of macropinocytosis.

Conclusions:

  • Understanding the varied mechanisms and regulation of macropinocytosis is essential for elucidating its physiological roles.
  • Further investigation is required to fully characterize macropinocytosis pathways and their functions in different cellular contexts and in vivo.
  • Comparative studies are key to advancing the field of macropinocytosis research.