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Characterization of surface markers of continuously growing murine resident macrophages
C D Muller1, Y Lombard, J Bartholeyns
1Université Louis Pasteur, Laboratoire de Chimie Enzymatique (UA CNRS 04 1182), Institut de Botanique, Strasbourg, France.
Abstract:
Conditions have been described which allow an in vitro indefinite multiplication of differentiated murine macrophages (Lombard et al: Biol Cell 53, 219, 1985). R. and MAY-1 cell lines, which were obtained, respectively, from mouse (Balb/c) spleen and resident peritoneal macrophages, have been further characterized. They present at their surface, besides the Mac-1 antigen and Fc-receptor, a mannose receptor which was characterized for its binding properties. This receptor is responsive for a specific phagocytosis of mannosylated particles, i.e., mannosylated latex beads or oil droplets containing mannosylated bovine serum albumin. Moreover, R and MAY-1 cells present an ectoenzyme profile (NAD+ glycohydrolase and nucleotide pyrophosphatase) similar to those of the corresponding resident macrophages.
Insights
Murine macrophage cell lines (R and MAY-1) were characterized for their surface receptors and enzyme profiles. These cells exhibit specific phagocytosis via a mannose receptor, similar to resident macrophages.
Area of Science:
- Immunology
- Cell Biology
- Macrophage Biology
Background:
- Differentiated murine macrophages can be cultured indefinitely in vitro.
- Characterization of specific macrophage cell lines is crucial for understanding their functions.
Purpose of the Study:
- To further characterize the R and MAY-1 murine macrophage cell lines.
- To investigate the surface receptors and ectoenzyme profiles of these cell lines.
Main Methods:
- Cell culture of murine spleen and peritoneal macrophages.
- Surface antigen and receptor analysis (Mac-1, Fc-receptor, mannose receptor).
- Phagocytosis assays using mannosylated particles.
- Ectoenzyme activity profiling (NAD+ glycohydrolase, nucleotide pyrophosphatase).
Main Results:
- R and MAY-1 cells express Mac-1 antigen, Fc-receptor, and a functional mannose receptor.
- The mannose receptor mediates specific phagocytosis of mannosylated particles.
- These cell lines share an ectoenzyme profile similar to resident macrophages.
Conclusions:
- R and MAY-1 cell lines are valuable models for studying differentiated murine macrophages.
- The mannose receptor plays a significant role in the phagocytic activity of these macrophages.
- The characterized properties support their utility in immunological research.