Related Experiment Video
Updated: Dec 12, 2025
06:16
Cryosectioning Method for Microdissection of Murine Colonic Mucosa
Published on: July 12, 2015
18.0K
Two histologic compartments in nonpolypoid conventional colon adenomas.
1Gastrointestinal Research Laboratory, Department of Pathology, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden.
Journal of Gastroenterology and Hepatology
|August 7, 2020
Summary
Colon adenomas have two compartments: dysplastic and nondysplastic. The nondysplastic compartment shows altered crypts with disorganized stem cells, suggesting early-stage somatic mutations in conventional adenomas.
Area of Science:
- Gastroenterology
- Cell Biology
- Oncology
Background:
- Nonpolypoid conventional adenomas feature two distinct tissue compartments.
- The lower, nondysplastic compartment with irregular crypts has been understudied.
- Recent research has begun to explore the biological characteristics of this nondysplastic compartment.
Purpose of the Study:
- To investigate the histological and biological attributes of the nondysplastic compartment in nonpolypoid conventional adenomas.
- To understand the role of stem cell reorganization and crypt alterations in adenoma development.
Main Methods:
- Histological examination of adenoma tissue.
- Analysis of proliferation markers, such as Ki67.
- Assessment of p53 expression and crypt fission patterns.
Main Results:
- The nondysplastic compartment exhibits crypts with irregular shapes and normal epithelium.
- Proliferating cell domains are haphazardly distributed within these crypts, indicating stem cell reorganization.
- Evidence of p53 upregulation and asymmetric crypt fission suggests histo-biological alterations and potential somatic mutations.
Conclusions:
- The nondysplastic compartment in conventional adenomas is histo-biologically altered, likely due to somatic mutations.
- Stem cell reorganization within irregular crypts is a key feature.
- Further research is needed to fully elucidate the molecular mechanisms of crypto-histogenesis in these adenomas.
Related Concept Videos
01:26Histology of the Large Intestine
2.5K
The large intestine, a vital component of the gastrointestinal tract, is structured with four main layers: the mucosa, submucosa, muscularis, and serosa. Each layer performs a distinct role in facilitating the smooth functioning of the large intestine.
The innermost mucosa layer comprises simple columnar epithelium, lamina propria, and muscularis mucosae. This layer is primarily populated with absorptive cells, tasked with water absorption, and goblet cells, responsible for secreting mucus to...
The innermost mucosa layer comprises simple columnar epithelium, lamina propria, and muscularis mucosae. This layer is primarily populated with absorptive cells, tasked with water absorption, and goblet cells, responsible for secreting mucus to...
2.5K
01:27Histology of the Small Intestine
3.0K
The small intestine exhibits a unique histological structure that significantly enhances its function in digestion and nutrient absorption. These structures include circular folds, villi, and various specialized cells that collectively facilitate the digestion of food.
The intestinal lining features transverse folds called circular folds, each housing fingerlike projections known as intestinal villi. These villi are covered by a layer of simple columnar epithelium, also referred to as...
The intestinal lining features transverse folds called circular folds, each housing fingerlike projections known as intestinal villi. These villi are covered by a layer of simple columnar epithelium, also referred to as...
3.0K
01:20Histology of the Gastrointestinal (GI) Tract
2.9K
The GI tract, from beginning to end, is made up of four continuous tissue layers that adjust their structure according to their specific roles. These layers, from innermost to outermost, are known as the mucosa, submucosa, muscularis, and serosa, which are continuous with the mesentery.
The mucosa is sometimes called a mucous membrane due to its mucus-secreting features. This membrane is composed of epithelium, which directly interacts with ingested substances, and the lamina propria, a layer...
The mucosa is sometimes called a mucous membrane due to its mucus-secreting features. This membrane is composed of epithelium, which directly interacts with ingested substances, and the lamina propria, a layer...
2.9K
01:23Renewal of Intestinal Stem Cells
3.0K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.0K
01:26Stomach Histology
2.5K
The stomach comprises several layers that work together to facilitate digestion and protect the organ. The outermost layer is called the serosa, which provides support and protection to the stomach. The muscularis externa layer is responsible for the mechanical breakdown of food by contracting and moving the stomach. The submucosa layer, located beneath the muscularis externa, contains connective tissue, blood vessels, nerves, and glands that secrete mucus and other substances essential for...
2.5K
01:20Compartment Models: Two-Compartment Model
6.7K
The two-compartment model divides the body into central and peripheral compartments to account for varying blood perfusion rates among organs and tissues, affecting drug distribution. The central compartment includes blood and highly perfused tissues with rapid drug distribution, while the peripheral compartment contains tissues with slower drug distribution. After a single IV bolus dose, the drug concentration is high in plasma and low in tissues. The drug distribution between compartments...
6.7K