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Published on: February 27, 2016
Reverse expression pattern of sirtuin-1 and histone deacetylase-9 in coronary artery disease
Laleh Heidari1, Sayyed Mohammad Hossein Ghaderian1,2, Milad Bastami3
1Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Insights
Reduced SIRT1 and elevated HDAC9 gene expression, along with specific methylation patterns, may serve as biomarkers for detecting coronary artery disease (CAD). These findings highlight potential diagnostic indicators for this cardiovascular condition.
Area of Science:
- Molecular Biology
- Genetics
- Cardiovascular Research
Background:
- Inflammation plays a role in coronary artery disease (CAD) pathogenesis.
- Sirtuin 1 (SIRT1) and Histone Deacetylase 9 (HDAC9) genes are implicated in inflammatory processes.
- Understanding the molecular underpinnings of CAD is crucial for developing diagnostic tools.
Purpose of the Study:
- To investigate the expression levels, methylation status, and genetic polymorphisms of SIRT1 and HDAC9 in patients with CAD.
- To determine if these genes can serve as potential biomarkers for CAD detection.
Main Methods:
- Real-Time PCR was used to quantify gene expression levels.
- High-Resolution Melting (HRM) analysis was employed to assess gene promoter methylation and identify polymorphisms.
- The study included 50 CAD patients and 50 healthy controls.
Main Results:
- SIRT1 expression was significantly reduced in CAD patients, while HDAC9 expression was significantly elevated (p < .001).
- SIRT1 gene promoter hypomethylation and HDAC9 gene promoter hypermethylation were observed in CAD patients.
- Specific genotypes of SIRT1 (CG+GG) and HDAC9 were associated with altered gene expression.
Conclusions:
- Expression changes in SIRT1 and HDAC9 genes present a potential biomarker for CAD detection.
- Aberrant methylation patterns of SIRT1 and HDAC9 promoters correlate with CAD.
- Genetic variations in SIRT1 and HDAC9 may influence their expression and contribute to CAD pathogenesis.
Background:
SIRT1 and HDAC 9 genes are related to inflammation and may contribute to the pathogenesis of coronary artery disease (CAD). We aimed to evaluate the expression level, methylation profile and polymorphisms of these genes in CAD patients.
Methods:
In this study, 50 CAD patients and 50 healthy individuals were recruited. The expression level change was evaluated using the TaqMan Real-Time PCR method. The methylation of genes promoter and genotyping of polymorphisms were evaluated by the HRM.
Results:
The expression level of SIRT1 was reduced while the HDAC9 expression level showed a significant elevation (p < .001). The SIRT1 gene promoter was hypomethylated and the HDAC9 gene promoter was hypermethylated in CAD patients. Also, CG + GG genotype in SIRT1 and both genotypes in the HDAC9 gene were associated with expression change.
Conclusions:
SIRT1 and HDAC9 genes, expression changes can be suggested as a potential biomarker for CAD detection.
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