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Gene Flow02:39

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Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
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Related Experiment Video

Updated: Dec 12, 2025

Establishing Pollination Requirements in Japanese Plum by Phenological Monitoring, Hand Pollinations, Fluorescence Microscopy and Molecular Genotyping
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Dynamic changes impact the plum pox virus population structure during leaf and bud development.

Yvette B Tamukong1, Tamara D Collum2, Andrew L Stone3

  • 1Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.

Virology
|August 8, 2020
PubMed
Summary

Plum pox virus (PPV) variants are more abundant in developing plum tissues. Viral population structure is influenced by host tissue type and seasonal development.

Keywords:
PhloemPopulation dynamicsTissue specificityVernalization

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Area of Science:

  • Plant Virology
  • Molecular Evolution
  • Next-Generation Sequencing

Background:

  • Plum pox virus (PPV) poses a significant global threat to stone fruit crops.
  • Woody perennial hosts offer a complex environment for viral evolution across seasons.

Purpose of the Study:

  • To investigate how seasonal host development influences PPV population structure.
  • To analyze PPV variants within different host tissues and developmental stages.

Main Methods:

  • Employed next-generation sequencing of ribosome-associated viral genomes (translatome).
  • Assessed PPV variants from phloem and whole leaf tissues across various plum developmental stages.

Main Results:

  • Translatome PPV variants were proportionally higher in developing buds and leaves with low infection levels.
  • Mature tissues with high infection levels showed proportionally fewer viral variants.
  • Distinct PPV variant groups were identified based on population frequency and tissue specificity.

Conclusions:

  • PPV population dynamics are significantly affected by host tissue type.
  • Seasonal host development plays a crucial role in shaping PPV variant populations.