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Updated: Dec 12, 2025
An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
Published on: December 3, 2020
Development and validation of a highly sensitive HPLC-MS/MS method for the QAP14, a novel potential anti-cancer
Yuchen Guo1, Qingyu Yao1, Daming Kong1
1Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery System, Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China.
Abstract:
QAP14 is a novel anti-cancer compound exhibiting good efficacy and safety in preclinical study. To investigate its pharmacokinetic properties, a rapid and sensitive HPLC-MS/MS method was developed and validated to quantify the concentration of QAP14 in rat plasma. QAP14 was separated on ZORBAX Eclipse XDB-C8 column with a gradient elution. Erlotinib was selected as internal standard and plasma samples were prepared by precipitation with acetonitrile. In the pharmacokinetic study, rats were treated with QAP14 at 2.4 mg/kg (i.v.) or 6 mg/kg (p.o.). This method provided good linearity in the range of 0.5-1000 ng/mL in rat plasma. The accuracy, precision, matrix effect, recovery, stability and carryover fulfilled the criteria. The pharmacokinetic profiles of QAP14 in rats were described by non-compartmental analysis. The oral bioavailability was 50.29 %. The assay is reliable and requires only little sample volume, and the pharmacokinetic properties of QAP14 in rats may provide reference for future studies.
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