Related Experiment Video

Updated: Dec 12, 2025

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
08:06

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy

Published on: February 14, 2016

8.2K

Mislocalization of TORC1 to Lysosomes Caused by KIF11 Inhibition Leads to Aberrant TORC1 Activity

Yoon-Gu Jang1,2, Yujin Choi1,2, Kyoungho Jun1,2

  • 1Institute of Molecular Biology and Genetics, Seoul National University, Seoul 08826, Korea.

Molecules and Cells
|August 8, 2020
PubMed

Insights

Microtubules and KIF11 motor protein control mTORC1

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Insulin and amino acids regulate mechanistic target of rapamycin complex 1 (mTORC1) signaling.
  • Amino acids induce mTORC1 localization to the lysosome for activation.
  • The precise mechanisms of mTORC1 lysosomal transport remain unclear.

Purpose of the Study:

  • To elucidate the mechanism of mTORC1 dissociation from lysosomes.
  • To investigate the role of microtubules and motor proteins in mTORC1 regulation.
  • To understand how amino acid availability controls mTORC1 activity.

Main Methods:

  • Investigated mTORC1 localization and activity under conditions of amino acid scarcity.
  • Utilized microtubule disruption and KIF11 knockdown experiments.
  • Performed mechanistic studies to identify protein interactions.

Main Results:

  • Microtubules and KIF11 are essential for mTORC1 dissociation from lysosomes during amino acid starvation.
  • Disruption of microtubules or KIF11 knockdown leads to sustained lysosomal localization of mTORC1.
  • mTORC1 interacts with the motor domain of KIF11, facilitating its movement along microtubules.

Conclusions:

  • Identified a novel regulatory mechanism for mTORC1 based on amino acid availability.
  • Demonstrated a new role for KIF11 and microtubules in controlling mTOR signaling.
  • mTORC1 movement from lysosomes is mediated by KIF11-dependent transport along microtubules.
Keywords:
DrosophilaKIF11lysosomemTORC1microtubule

More Related Videos

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag08:55

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Published on: December 14, 2017

15.8K
Aip1p Dynamics Are Altered by the R256H Mutation in Actin08:57

Aip1p Dynamics Are Altered by the R256H Mutation in Actin

Published on: July 30, 2014

8.3K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
Destabilization of Microtubules01:45

Destabilization of Microtubules

The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
3.3K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.0K
Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
5.8K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.2K
Lysosomal Hydrolases01:22

Lysosomal Hydrolases

Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
4.3K