Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Properties of washed human platelets.

R L Kinlough-Rathbone, J F Mustard, M A Packham

    Thrombosis and Haemostasis
    |April 30, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Characteristics, Origins, and Biosignature Preservation Potential of Carbonate-Bearing Rocks Within and Outside of Jezero Crater.

    Journal of geophysical research. Planets·2021
    Same author

    Earth-like Habitable Environments in the Subsurface of Mars.

    Astrobiology·2021
    Same author

    Hearing in the Mind's Ear: A PET Investigation of Musical Imagery and Perception.

    Journal of cognitive neuroscience·2013
    Same author

    Changes in Phosphoinositides in Rabbit Platelets during Clot Formation. Comparison of Platelets Stimulated by ADP or by Thrombin in the Presence of Polymerising Fibrin.

    Platelets·2010
    Same author

    Ontario's Mustard Report: Its Implications For Family Physicians.

    Canadian family physician Medecin de famille canadien·2010
    Same author

    Closing remarks / conclusion.

    Canadian Medical Association journal·2010

    The suspending medium significantly impacts how washed human platelets respond to various aggregating agents. Platelet aggregation and release depend on factors like calcium, magnesium, and protein content in the medium.

    Area of Science:

    • Hematology
    • Cell Biology
    • Biochemistry

    Background:

    • Washed human platelets require specific conditions to maintain function.
    • Previous studies established platelet aggregation with ADP in albumin-containing Tyrode solution.

    Purpose of the Study:

    • To investigate the effects of different aggregating and release-inducing agents on washed human platelets.
    • To determine how the composition of the resuspending medium influences platelet responses.

    Main Methods:

    • Washed human platelets were resuspended in various modified Tyrode solutions.
    • Platelet aggregation and granule release were measured in response to collagen, arachidonate, thrombin, ADP, ristocetin, serotonin, adrenaline, immune serum globulin, ionophore A23187, phytohaemagglutinin, and polylysine.
    • Experiments were conducted in media with varying concentrations of calcium, magnesium, and protein.

    Related Experiment Videos

    Main Results:

    • Collagen, arachidonate, thrombin, immune serum globulin, ionophore A23187, and phytohaemagglutinin induced aggregation and release.
    • Serotonin caused shape change only; adrenaline response was variable.
    • Ristocetin required plasma; polylysine caused adherence with minimal release.
    • Medium composition (Ca2+, Mg2+, protein) modulated responses to ADP, collagen, thrombin, and polylysine, affecting aggregation and release.
    • Protein-free medium led to extensive lysis with polylysine.

    Conclusions:

    • The resuspending medium's composition critically affects washed human platelet function.
    • Specific ions (Ca2+, Mg2+) and proteins influence platelet aggregation and release pathways.
    • Understanding these medium-dependent responses is crucial for in vitro platelet studies.