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Updated: Dec 12, 2025

Spatial Temporal Analysis of Fieldwise Flow in Microvasculature

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Published on: November 18, 2019

Cortex-wide microcirculation mapping with ultrafast large-field multifocal illumination microscopy.

Zhenyue Chen¹, Quanyu Zhou¹, Johannes Rebling¹

¹Institute for Biomedical Engineering and Institute of Pharmacology and Toxicology, University of Zurich and ETH Zurich, Zurich, Switzerland.

Journal of Biophotonics

|August 8, 2020

Summary

This summary is machine-generated.

Large-field multifocal illumination (LMI) microscopy now achieves 200 Hz imaging of mouse brains. This advanced technique enables real-time observation of microcirculation and particle tracking across the entire cortex.

Keywords:

brain dynamics cerebral vasculature fluorescence microscopy in vivo tracking microcirculation ultrafast imaging

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Area of Science:

Neuroscience
Biomedical Optics
Microscopy

Background:

Transcranial observation of mouse brain dynamics requires high spatiotemporal resolution over a large field-of-view.
Existing techniques face limitations in simultaneously achieving capillary-level resolution and centimeter-scale imaging.
Large-field multifocal illumination (LMI) fluorescence microscopy offers potential for advanced brain dynamics studies.

Purpose of the Study:

To develop and validate an accelerated LMI technique for enhanced transcranial in vivo imaging.
To achieve high frame rates and resolutions for observing mouse brain microcirculation and cellular dynamics.
To demonstrate the capability of the new LMI scheme for large-scale functional neuroimaging.

Main Methods:

Implementation of an acceleration scheme for LMI utilizing raster scanning of a lattice pattern.
Integration of a parallel camera exposure scheme to boost imaging speed.
Acquisition of 200 Hz frame rates over a 12x12 mm^2 field-of-view with 7.5 μm spatial resolution.

Main Results:

Demonstration of real-time transcranial in vivo particle tracking across the entire mouse cortex.
Successful imaging of microcirculation dynamics with unprecedented spatiotemporal resolution.
Validation of LMI's superior performance compared to other existing imaging techniques.

Conclusions:

The accelerated LMI technique significantly enhances the capabilities for observing mouse brain dynamics.
This advancement provides a powerful tool for investigating cerebrovascular function, cell tracking, and large-scale functional neuroimaging.
LMI offers unique potential for neuroscience research due to its unmatched combination of resolution and field-of-view.