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Updated: Dec 12, 2025
Rescue of Recombinant Newcastle Disease Virus from cDNA
Published on: October 11, 2013
The 3D8 single chain variable fragment protein suppresses Newcastle disease virus transmission in transgenic chickens
Sung June Byun1, Hoonsung Choi1, Shanmugam Sureshkumar1
1Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, 55365, Wanju-gun, Republic of Korea.
Background:
The 3D8 single chain variable fragment (scFv) is a mini-antibody sequence that exhibits independent nuclease activity against all types of nucleic acids. In this research, crossing a 3D8 scFv G1 transgenic rooster with wild-type hens produced 3D8 scFv G2 transgenic chickens to evaluate suppression of viral transmission.
Result:
The transgenic chickens were identified using genomic PCR and immunohistochemistry. To evaluate Newcastle disease virus (NDV) protection conferred by 3D8 scFv expression, transgenic, non-transgenic, and specific pathogen-free (SPF) chickens were challenged with virulent NDV by direct injection or aerosol exposure. The three groups of chickens showed no significant differences (p < 0.05) in mean death time after being directly challenged with NDV; however, in contrast to chickens in the non-transgenic and SPF groups, chickens in the transgenic group survived after aerosol exposure. Although the transgenic chickens did not survive after direct challenge, we found that the chickens expressing the 3D8 scFv survived aerosol exposure to NDV.
Conclusions:
Our finding suggest that the 3D8 scFv could be a useful tool to prevent chickens from spreading NDV and control virus transmission.
