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Updated: Dec 12, 2025
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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NOTCH pathway activation in infantile hemangiomas.
Haihong Zhang1, Ting Wei1, Adam Johnson1
1Department of Otolaryngology and Head and Neck Surgery, University of Arkansas for Medical Sciences and Arkansas Children's Hospital, Little Rock, Ark.
Journal of Vascular Surgery. Venous and Lymphatic Disorders
|August 9, 2020
Summary
NOTCH signaling is crucial for infantile hemangioma (IH) development. Its activation is high in proliferating IHs but decreases with involution and propranolol treatment, indicating its role in IH pathogenesis.
Area of Science:
- Vascular Biology
- Dermatology
- Molecular Signaling
Background:
- Infantile hemangioma (IH) is a common vascular tumor in infants.
- The underlying molecular mechanisms driving IH angiogenesis are not fully understood.
- NOTCH signaling is a key pathway involved in cell differentiation and tissue development.
Purpose of the Study:
- To investigate the role of NOTCH signaling pathway activation in the angiogenic process of infantile hemangioma (IH).
- To compare NOTCH pathway component expression in different stages of IH and in response to propranolol treatment.
Main Methods:
- Tissue samples from 55 patients with infantile hemangioma (proliferating, stationary, involuting, propranolol-treated) and normal skin were analyzed.
- Western blotting and reverse transcription-polymerase chain reaction were used to quantify NOTCH pathway components.
Main Results:
- Proliferating IH tissues showed significantly higher messenger RNA and protein levels of NOTCH receptors, ligands, and MAML1 compared to normal skin.
- Expression of NOTCH receptor ligands was markedly reduced in involuting and propranolol-treated IHs.
Conclusions:
- NOTCH signaling pathway activation is significantly altered during the natural course and treatment of infantile hemangiomas.
- These findings suggest a critical role for NOTCH signaling in the pathogenesis and progression of IH.
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