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Understanding the interaction between triclocarban and denitrifiers.

Dongbo Wang1, Lingjuan Tao1, Jingnan Yang1

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Summary

Triclocarban (TCC) is removed by denitrifying sludge primarily through adsorption. However, long-term exposure to TCC negatively impacts sludge properties and significantly reduces denitrification efficiency and microbial diversity.

Keywords:
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Area of Science:

  • Environmental Science
  • Microbiology
  • Wastewater Treatment

Background:

  • Triclocarban (TCC) is widely used, leading to its release into aquatic environments.
  • Denitrifying cultures are crucial for wastewater treatment but their interaction with TCC is not well understood.

Purpose of the Study:

  • To investigate the removal of TCC by denitrifying sludge.
  • To assess the impact of TCC on the performance and properties of denitrifying cultures.

Main Methods:

  • Experimental removal of TCC by denitrifying sludge at varying concentrations.
  • Mass balance analysis to determine removal mechanisms (adsorption vs. biodegradation).
  • Assessment of sludge physicochemical properties and denitrification efficiency under short-term and long-term TCC exposure.
  • High-throughput sequencing for microbial community analysis.

Main Results:

  • 71.2%-79.4% TCC removal was achieved by denitrifying sludge, predominantly via non-homogeneous multilayer adsorption.
  • Short-term TCC exposure did not affect sludge properties, but long-term exposure deteriorated settleability, dewaterability, flocculability, and hydrophobicity.
  • A TCC concentration of 20 mg/L decreased denitrification efficiency by 70% in the long term.
  • TCC exposure increased extracellular polymeric substances (proteins) and decreased denitrifiers' activity.
  • Microbial community diversity and the abundance of key denitrifier genera were reduced by TCC.

Conclusions:

  • Denitrifying sludge can remove TCC mainly through adsorption.
  • Long-term TCC exposure poses a significant risk to wastewater treatment processes by impairing sludge performance and microbial community structure.
  • Further research is needed to understand the long-term ecological effects of TCC in aquatic environments.