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Updated: Dec 12, 2025
09:57
Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach
Published on: December 17, 2016
6.9K
The proteasome controls ESCRT-III-mediated cell division in an archaeon
Gabriel Tarrason Risa1, Fredrik Hurtig2, Sian Bray3
1MRC-Laboratory for Molecular Cell Biology, University College London (UCL), London, UK.
Science (New York, N.Y.)
|August 9, 2020
Summary
The archaeal proteasome regulates cell division in Sulfolobus acidocaldarius by degrading the ESCRT-III homolog CdvB, which allows constriction of the division ring and cell separation. This reveals a conserved proteasome role in cell cycle control.
Area of Science:
- Microbiology
- Cell Biology
- Biochemistry
Background:
- Sulfolobus acidocaldarius, an archaeon, exhibits a eukaryote-like cell cycle despite lacking canonical cell cycle regulators.
- Understanding archaeal cell division mechanisms provides insights into the evolution of eukaryotic cell cycles.
Purpose of the Study:
- To investigate the mechanism of cell division in S. acidocaldarius.
- To identify key regulators of the transition between cell cycles in archaea.
Main Methods:
- Proteasome activity assays in S. acidocaldarius.
- Identification and characterization of ESCRT-III homologs involved in cell division.
- Analysis of CdvB protein degradation and its effect on cell division.
Main Results:
- The archaeal proteasome plays a crucial role in regulating cell cycle transitions.
- The ESCRT-III homolog CdvB is a target of proteasomal degradation.
- Degradation of CdvB is essential for triggering cell division by enabling ESCRT-III ring constriction.
Conclusions:
- A minimal mechanism for ESCRT-III-mediated membrane remodeling during cell division is proposed.
- The proteasome is conserved in regulating cell cycle control in both archaea and eukaryotes.
- This study highlights the archaeal proteasome's role in S. acidocaldarius cell division.
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