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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
A study on the response of FRET based DNA aptasensors in intracellular environment
Shreya Ghosh1, Yinghua Chen2, Jesvin Sebastian3
1Department of Bioengineering, University of Illinois at Chicago, 851 South Morgan Street (SEO 218), Chicago, IL, 60607, USA. sghosh9@uic.edu.
Abstract:
This paper presents a study of the response of FRET based DNA aptasensors in the intracellular environment. Herein, we extend previous studies of aptasensors functioning in the extracellular environment to detection of antigens in the intracellular environment. An essential step in this research is the use of a novel means of achieving the endocytosis of aptasensors. Specifically, it is demonstrated that functioning aptasensors are successfully endocytosed by functionalizing the aptasensors with endocytosis-inducing DSS peptides.

