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Long Non-Coding RNA TP73-AS1 Promotes the Development of Lung Cancer by Targeting the miR-27b-3p/LAPTM4B Axis

Qingfeng Jiang1, Wenqun Xing1, Jinhua Cheng1

  • 1Department of Thoracic Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, People's Republic of China.

Oncotargets and Therapy
|August 9, 2020
PubMed
Abstract

Insights

Long non-coding RNA TP73-AS1 promotes lung cancer progression by regulating the miR-27b-3p/LAPTM4B axis. This finding suggests TP73-AS1 as a potential diagnostic and therapeutic target for lung cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • The specific function of TP73 antisense RNA 1T (TP73-AS1) in lung cancer remains largely unexplored.

Purpose of the Study:

  • To investigate the role of TP73-AS1 in lung cancer progression.
  • To elucidate the molecular mechanism underlying TP73-AS1's function in lung cancer, including its interaction with microRNA-27b-3p (miR-27b-3p) and lysosomal-associated protein transmembrane-4 Beta (LAPTM4B).

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) to measure gene expression.
  • Cellular assays (MTT, Annexin V-FITC/PI, Transwell) to assess proliferation, apoptosis, migration, and invasion.
  • In vivo tumor xenograft models.
  • Luciferase reporter assays to confirm molecular interactions.
  • Western blot analysis for protein expression.

Main Results:

  • TP73-AS1 expression was significantly upregulated in lung cancer tissues and cells.
  • TP73-AS1 depletion inhibited lung cancer cell growth and metastasis in vitro and tumor growth in vivo.
  • TP73-AS1 functions as an endogenous sponge for miR-27b-3p, with an inverse correlation observed between their expression.
  • miR-27b-3p directly targets LAPTM4B, and this axis is co-regulated by TP73-AS1.

Conclusions:

  • TP73-AS1 promotes lung cancer progression via the miR-27b-3p/LAPTM4B regulatory axis.
  • TP73-AS1 represents a potential biomarker for lung cancer diagnosis and a therapeutic target.
Keywords:
LAPTM4BTP73-AS1lung cancermetastasismiR-27b-3pproliferation

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