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Related Experiment Video

Updated: Dec 12, 2025

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation

Published on: April 27, 2021

Mast Cell Purification Protocols.

Jasmine Farrington¹, Elizabeth P Seward², Peter T Peachell³

¹Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield, UK.

Methods in Molecular Biology (Clifton, N.J.)

|August 9, 2020

Summary

This summary is machine-generated.

This study details methods for isolating human lung mast cells. These techniques are adaptable for mast cell isolation from various tissues, acknowledging cellular heterogeneity.

Keywords:

CD117 Dynabeads®Mast cells

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Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

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Last Updated: Dec 12, 2025

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation

Published on: April 27, 2021

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

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Published on: September 29, 2019

Area of Science:

Immunology
Cell Biology

Background:

Mast cells exhibit significant heterogeneity depending on their tissue of origin.
Established methods for mast cell isolation exist but require refinement.

Purpose of the Study:

To provide detailed protocols for the isolation and purification of human lung mast cells.
To highlight the general applicability of these methods to mast cell isolation from diverse tissues.

Main Methods:

Detailed protocols for mast cell isolation from human lung tissue.
Discussion of method adaptability for other tissue sources.

Main Results:

Refined methodologies for obtaining purified human lung mast cells.
Demonstration of the general applicability of the described techniques.

Conclusions:

The provided methods enable effective isolation of human lung mast cells.
These techniques are broadly applicable for studying tissue-specific mast cell heterogeneity.