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Updated: Dec 12, 2025

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
Published on: August 9, 2019
Generation and Culture of Peripheral CD34+ Stem Cell-Derived Mast Cells (PSCMCs)
Yanyan Luo1, Stefan Frischbutter1, Jörg Scheffel1
1Department of Dermatology and Allergy, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Abstract:
Mast cells (MCs) are long-living tissue-resident cells that play an important role in inflammatory and allergic reactions. In vitro models of mast cell functions have allowed better understanding of the function of mast cells and mast cell-mediated disorders. In this unit, we describe a protocol used for the generation and culture of peripheral CD34+ stem cell-derived mast cells (PSCMCs). It provides a useful tool for the investigation of the biology of human MCs in vitro.

