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Basophil Activation Test for Allergy Diagnosis
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Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques.

Chris H Bridts1, Vito Sabato1, Christel Mertens1

  • 1Department of Immunology, Allergology, Rheumatology, University of Antwerp, Antwerp University Hospital, Antwerp, Belgium.

Methods in Molecular Biology (Clifton, N.J.)
|August 9, 2020
PubMed
Summary

Flow cytometry aids allergy diagnosis by measuring basophil activation markers. Two new methods detect surface marker changes and histamine release, offering insights into allergic responses.

Keywords:
Basophil activation testsCD203cCD63HistaFlow

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Area of Science:

  • Immunology
  • Allergy Diagnostics
  • Flow Cytometry

Background:

  • Flow cytometry is used for allergy diagnosis by quantifying changes in basophil surface markers.
  • Allergen exposure triggers basophils to release mediators and upregulate markers like CD63 and CD203c.
  • These changes are detectable via multicolor flow cytometry using monoclonal antibodies.

Purpose of the Study:

  • To describe two flow cytometry-based protocols for assessing basophil activation.
  • To enable detection of surface marker activation (Method 1).
  • To enable detection of changes in intragranular histamine (Method 2).

Main Methods:

  • Development of two distinct flow cytometry protocols.
  • Method 1: Detection of surface marker expression changes on activated basophils.
  • Method 2: Quantification of intragranular histamine levels in basophils.

Main Results:

  • The described protocols allow for the assessment of basophil activation.
  • Method 1 quantifies surface marker upregulation, reflecting early activation events.
  • Method 2 measures histamine content, indicating mediator release.

Conclusions:

  • These flow cytometry methods provide complementary approaches to evaluate basophil activation in allergic conditions.
  • The protocols enhance the diagnostic capabilities for allergy testing.
  • Both surface marker expression and mediator content offer valuable insights into basophil function during allergic responses.