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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Multifunctional aggregation-based fluorescent probe for visualizing intracellular calcium dynamic fluctuations.

Hang Su¹, Ping Ping Hu², Xiao Li Jiang¹

¹Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, Chongqing, 401331, China.

Analytical and Bioanalytical Chemistry

|August 9, 2020

Summary

This summary is machine-generated.

Researchers developed novel nanoparticles for detecting calcium ions (Ca2+), crucial for cell signaling. These nanoparticles offer enhanced fluorescence and stability, enabling precise biological sensing and imaging of cells with abnormal calcium levels.

Keywords:

Bio-imaging CPM@Cur NPs Ca2+ sensing Förster resonance energy transfer Sarco/endoplasmic reticulum Ca2+ ATPase

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Functional Calcium Imaging in Developing Cortical Networks

Functional Calcium Imaging in Developing Cortical Networks

Published on: October 22, 2011

Area of Science:

Biomedical Engineering
Nanotechnology
Analytical Chemistry

Background:

Calcium ion (Ca2+) is a vital second messenger in biological systems.
Dysfunctional Ca2+ regulation is linked to various diseases.
Developing sensitive and specific Ca2+ detection methods is crucial for biological research and diagnostics.

Purpose of the Study:

To develop a simple and effective method for encapsulating a coumarin-based Ca2+ probe (CPM) into nanoparticles (NPs).
To enhance the optical properties of the Ca2+ probe using Förster resonance energy transfer (FRET) with curcumin.
To evaluate the utility of these engineered nanoparticles for biological sensing and intracellular Ca2+ imaging.

Main Methods:

Encapsulation of a coumarin-based Ca2+ probe (CPM) into nanoparticles (NPs).
Co-encapsulation of CPM with curcumin (FRET donor and acceptor) to create CPM@Cur NPs.
Characterization of NP fluorescence properties (excitation/emission wavelengths, sensitivity, LOD).
Assessment of NP cytotoxicity, stability, and application in cell discrimination.

Main Results:

CPM NPs exhibited blue fluorescence with maximum excitation at 365 nm and emission at 450 nm.
CPM NPs showed significant fluorescence enhancement for Ca2+ with a limit of determination (LOD) of 0.04 μM.
Co-encapsulation with curcumin yielded bright green CPM@Cur NPs with improved optical properties (large Stokes shift, narrow emission bandwidth).
CPM NPs and CPM@Cur NPs demonstrated low cytotoxicity and excellent stability.
Successfully discriminated primary aortic smooth muscle cells with abnormal Ca2+ homeostasis from controls.

Conclusions:

Developed a novel strategy for synthesizing bright nanoparticles for Ca2+ sensing.
CPM@Cur NPs offer enhanced fluorescence signals and reduced background interference for Ca2+ monitoring.
These nanoparticles show great potential for intracellular Ca2+ imaging and diagnosing diseases related to calcium imbalance.