Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

4.5K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

8.1K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.1K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

10.1K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Factors influencing outcomes of older children with medulloblastoma over 15 years in Peru, a resource-limited setting.

Pediatric blood & cancer·2022
Same journal

Peptidomics in the Spotlight: Advanced Sample Treatment Techniques and Analytical Insights.

Advances in experimental medicine and biology·2026
Same journal

Methods for the Investigation of Protein-Ligands Interactions.

Advances in experimental medicine and biology·2026
Same journal

Sample Preparation Strategies for Microbial Cell Surface Proteomics: Integrating Shaving and Shotgun Approaches.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Sample Preparation for the Petroleum Industry: A Biocorrosion Case Study.

Advances in experimental medicine and biology·2026
Same journal

Proteomic and Functional Comparison of Extracellular Vesicles from Wild-Type and Lyn-Deficient Stromal Cells.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Analysis of Histone Sequence Variants and Post-translationally Modified Forms.

Advances in experimental medicine and biology·2026
See all related articles

Related Experiment Video

Updated: Dec 12, 2025

Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay
07:44

Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay

Published on: March 12, 2018

10.4K

Targetable Intercellular Signaling Pathways Facilitate Lung Colonization in Osteosarcoma.

James Brandon Reinecke1, Ryan David Roberts2

  • 1Department of Pediatrics, Nationwide Children's Hospital and The Ohio State University, Columbus, OH, USA.

Advances in Experimental Medicine and Biology
|August 9, 2020
PubMed
Summary
This summary is machine-generated.

Osteosarcoma metastasis to the lung is driven by cell-specific adaptations and lung interactions. Aberrant ΔNp63 expression promotes IL6 and CXCL8 production, facilitating osteosarcoma lung colonization.

Keywords:
CXCL8Cell-autonomous mechanismIL6Intercellular signalingLung colonizationMetastasisMetastatic bottleneckOsteosarcomaTumor microenvironmentTumor-host interactionsΔNp63

More Related Videos

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells
08:47

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

Published on: August 21, 2012

17.6K
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

10.8K

Related Experiment Videos

Last Updated: Dec 12, 2025

Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay
07:44

Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay

Published on: March 12, 2018

10.4K
Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells
08:47

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

Published on: August 21, 2012

17.6K
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

10.8K

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Metastasis Research

Background:

  • Osteosarcoma patient outcomes critically depend on lung metastasis development.
  • The lung's specific microenvironment supports osteosarcoma cell survival and proliferation.
  • Mechanisms of osteosarcoma lung metastasis are incompletely understood but involve cell-autonomous and tumor-host interactions.

Purpose of the Study:

  • To elucidate key molecular pathways driving osteosarcoma lung metastasis.
  • To highlight the integration of cell-autonomous adaptations and lung-specific tumor-host interactions.
  • To identify potential therapeutic targets for preventing osteosarcoma lung metastasis.

Main Methods:

  • Review of existing literature on osteosarcoma metastasis mechanisms.
  • Focus on cell-autonomous adaptations including epigenetic reprogramming.
  • Analysis of tumor-host signaling pathways involving lung epithelium, stem cells, and immunity.

Main Results:

  • Osteosarcoma lung metastasis involves epigenetic reprogramming enabling resistance to apoptosis and ER stress.
  • Tumor cells exploit intercellular signaling networks for survival and niche creation.
  • Aberrant ΔNp63 expression in tumor cells induces IL6 and CXCL8 production upon lung epithelial cell interaction.

Conclusions:

  • Tumor-derived IL6 and CXCL8 mediate autocrine, paracrine, and immune-related interactions that promote lung metastasis.
  • These identified pathways are potentially targetable with existing therapeutics.
  • Further research into metastasis mechanisms is crucial for improving osteosarcoma patient outcomes.