Related Experiment Video

Updated: Dec 12, 2025

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.0K

MiR-203 regulates proliferation and apoptosis of ovarian cancer cells by targeting SOCS3

H-P Liu1, Y Zhang, Z-T Liu

  • 1Department of Reproductive Medicine Center, The 960th Hospital of the PLA Joint Logistics Support Force, Jinan, Shandong, China.

European Review for Medical and Pharmacological Sciences
|August 9, 2020
PubMed

Insights

This article on miR-203, SOCS3, and ovarian cancer cell regulation has been withdrawn due to suspected research misconduct. The publisher retracted the study after authors failed to verify data originality.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Ovarian cancer is a significant global health challenge.
  • MicroRNAs (miRNAs) play crucial roles in cancer development and progression.
  • Specific miRNAs are being investigated as potential therapeutic targets in ovarian cancer.

Purpose of the Study:

  • To investigate the role of microRNA-203 (miR-203) in regulating the proliferation and apoptosis of ovarian cancer cells.
  • To identify Signal Transducer and Activator of Transcription 3 (STAT3) pathway-related protein, Suppressor of Cytokine Signaling 3 (SOCS3), as a direct target of miR-203.
  • To elucidate the molecular mechanism by which miR-203 influences ovarian cancer cell behavior.

Main Methods:

  • Cell culture of human ovarian cancer cell lines.
  • Quantitative real-time polymerase chain reaction (qRT-PCR) to measure miR-203 and SOCS3 expression.
  • Western blotting to assess protein levels of SOCS3 and apoptosis-related markers.
  • Cell proliferation assays (e.g., MTT assay).
  • Apoptosis assays (e.g., flow cytometry with Annexin V/propidium iodide staining).

Main Results:

  • MiR-203 expression was found to be downregulated in ovarian cancer tissues and cell lines.
  • Overexpression of miR-203 significantly inhibited ovarian cancer cell proliferation.
  • MiR-203 overexpression induced apoptosis in ovarian cancer cells.
  • SOCS3 was identified as a direct target of miR-203, with miR-203 negatively regulating SOCS3 expression.
  • Inhibition of SOCS3 partially mimicked the effects of miR-203 overexpression on cell proliferation and apoptosis.

Conclusions:

  • MiR-203 functions as a tumor suppressor in ovarian cancer.
  • The miR-203/SOCS3 axis plays a critical role in regulating ovarian cancer cell proliferation and apoptosis.
  • MiR-203 may represent a potential therapeutic target for ovarian cancer treatment.

More Related Videos

Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-107:59

Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1

Published on: December 5, 2020

2.9K
Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

7.1K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.6K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
37.9K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.5K