Related Experiment Videos
Granulocyte/macrophage colony-stimulating factor induces interleukin 1 production by human polymorphonuclear
A Lindemann1, D Riedel, W Oster
1Department of Hematology, University of Mainz, FRG.
Abstract:
The purpose of this study was to determine whether human polymorphonuclear neutrophils (PMN), which share a common cell lineage with macrophages, could produce factors such as IL-1. We show by Northern blot analysis and bioassays that PMN can be induced to accumulate mRNA specific for IL-1 alpha and IL-1 beta indistinguishable in size from IL-1 mRNA synthesized by activated human macrophages and consequently to release IL-1-like activity in their culture supernatants, that could be neutralized by a mAb to IL-1. The granulocyte-macrophage colony-stimulating factor was identified as a major physiologic inducer for PMN-IL-1.
Insights
Human polymorphonuclear neutrophils (PMN) can produce Interleukin-1 (IL-1) alpha and beta. Granulocyte-macrophage colony-stimulating factor is a key inducer of this PMN-derived IL-1.
Area of Science:
- Immunology
- Cell Biology
Background:
- Human polymorphonuclear neutrophils (PMN) share a common cell lineage with macrophages.
- Macrophages are known producers of Interleukin-1 (IL-1).
Purpose of the Study:
- To investigate if human PMN can produce IL-1.
- To identify potential inducers of IL-1 production in PMN.
Main Methods:
- Northern blot analysis to detect IL-1 mRNA accumulation in PMN.
- Bioassays to measure IL-1-like activity in PMN culture supernatants.
- Monoclonal antibody (mAb) neutralization assays to confirm IL-1 identity.
Main Results:
- PMN were induced to accumulate mRNA for IL-1 alpha and IL-1 beta.
- The size of PMN-derived IL-1 mRNA was indistinguishable from that of macrophages.
- PMN released IL-1-like activity neutralized by anti-IL-1 mAb.
- Granulocyte-macrophage colony-stimulating factor (GM-CSF) was identified as a major physiological inducer.
Conclusions:
- Human PMN are capable of producing IL-1 alpha and IL-1 beta.
- GM-CSF is a significant physiological inducer of IL-1 production in PMN.