Related Experiment Video

Updated: Dec 12, 2025

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer
07:13

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

Published on: March 26, 2014

23.8K

Targeting RNA helicase DHX33 blocks Ras-driven lung tumorigenesis in vivo

Xingshun Wang1,2, Weimin Feng1, Cheng Peng1,2

  • 1Department of Biology, Southern University of Science and Technology, Shenzhen, China.

Cancer Science
|August 9, 2020
PubMed

Insights

Targeting DHX33, a Ras downstream effector, inhibits tumor development in Ras-mutant lung cancer. This study reveals DHX33

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Ras mutations drive 30% of non-small cell lung cancers (NSCLC).
  • No effective drugs currently target Ras for NSCLC treatment.
  • DHX33 was previously identified as a Ras downstream effector promoting cell growth.

Purpose of the Study:

  • To investigate the role of DHX33 in Ras-driven lung cancer development in vivo.
  • To explore DHX33 as a potential therapeutic target for Ras-mutant NSCLC.

Main Methods:

  • Utilized a K-Ras (G12D);DHX33 (lox/lox) mouse model.
  • Performed genetic ablation of DHX33.
  • Analyzed gene expression changes following DHX33 ablation.

Main Results:

  • Genetic ablation of DHX33 significantly inhibited tumor development.
  • DHX33 ablation altered the expression of approximately 2000 genes.
  • Affected genes are critical for cell cycle, apoptosis, glycolysis, Wnt signaling, and cell migration.

Conclusions:

  • DHX33 plays a pivotal role in Ras-driven lung cancer development.
  • Pharmacological targeting of DHX33 presents a feasible strategy for treating Ras-mutant lung cancers.
Keywords:
DHX33RNA helicaseRaslung canceroncogene

More Related Videos

Inducible and Reversible Dominant-negative DN Protein Inhibition08:35

Inducible and Reversible Dominant-negative DN Protein Inhibition

Published on: January 7, 2019

8.6K
DNA Vector-based RNA Interference to Study Gene Function in Cancer13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

20.9K

Related Concept Videos

The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.9K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
5.0K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.1K