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LC-MS/MS Determination of Apigenin in Rat Plasma and Application to Pharmacokinetic Study
Shixing Zhu1, Jiayuan Zhang1, Zhihua Lv1
1School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Background:
Apigenin, a natural plant flavone, has been shown to possess a variety of biological properties.
Objective:
In this report, a highly selective and sensitive LC-MS/MS method was developed and validated for the determination of apigenin in rat plasma.
Methods:
Analysts were separated on the HSS T3 column (1.8 μm 2.1×100 mm) using acetonitrile and 0.1% formic acid in 2mM ammonium acetate buffer at a supply rate of 0.200 mL/min as eluent in gradient model.
Results:
Plasma samples were treated by protein precipitation using acetonitrile for the recovery ranging from 86.5% to 90.1% for apigenin. The calibration curves followed linearity in the concentration range of 0.50-500ng/mL. The inter-day and intra-day precisions at different QC levels within 13.1% and the accuracies ranged from -10.6% to 8.6%.
Conclusion:
The assay has been successfully applied to the pharmacokinetic study of apigenin in rats.
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