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Technical Note: Assessment of two methods for estimating bone ash in pigs.

Madie R Wensley¹, Carine M Vier¹, Jordan T Gebhardt¹

¹Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, KS.

Journal of Animal Science

|August 10, 2020

Summary

Comparing bone processing methods for swine diets, this study found that defatting bones increased bone ash percentage. However, both defatted and non-defatted methods effectively detected dietary phosphorus differences in nursery pigs.

Keywords:

bone ash ether extraction phosphorus pigs

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Area of Science:

Animal Nutrition
Swine Physiology
Bone Metabolism

Background:

Accurate assessment of bone mineralization is crucial for evaluating swine diets.
Dietary phosphorus (P) levels significantly impact bone health in pigs.
Bone processing methods may influence the accuracy of bone ash analysis.

Purpose of the Study:

To compare two bone processing methods (defatted vs. non-defatted) for their influence on treatment differences.
To evaluate the ability of each processing method to detect changes in bone ash percentage due to dietary P.
To determine the optimal bone processing method for nursery and finishing pigs.

Main Methods:

Three experiments utilized nursery and finishing pigs fed varying available phosphorus levels.
Bones (fibulas or metacarpals) were collected and processed using either defatting (ether extraction) or non-defatting methods.
Percentage bone ash was determined for both processing methods to assess bone mineralization.

Main Results:

Defatted bones showed significantly higher bone ash percentage compared to non-defatted bones in both nursery and finishing pigs.
No significant method × treatment interactions were observed in nursery pigs, indicating processing method did not affect treatment detection.
A minimal linear interaction was detected in finishing pigs' percentage bone ash, attributed to increased variation in non-defatted samples.

Conclusions:

Both defatted and non-defatted bone processing methods are suitable for assessing bone ash and dietary P effects in nursery pigs.
The defatted bone processing method is preferred for grow-finish pigs due to reduced variation in bone ash analysis.
Bone processing method does not impede the detection of dietary P treatment differences in swine.