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Lysobacter gilvus sp. nov., isolated from activated sludge.

Jianyi Xu1, Mengyao Sheng1, Zhou Yang1

  • 1Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.

Archives of Microbiology
|August 10, 2020
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Summary

A novel aerobic bacterium, Lysobacter gilvus sp. nov. strain HX-5-24T, was discovered in agricultural plant sludge. Phylogenetic and genomic analyses confirm it represents a new species within the Lysobacter genus.

Keywords:
16S rRNA gene sequenceLysobacter gilvus sp. nov.Polyphasic taxonomySludge

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Area of Science:

  • Microbiology
  • Bacteriology
  • Genomics

Background:

  • The genus Lysobacter encompasses diverse bacteria known for various metabolic capabilities.
  • Microbial diversity in industrial wastewater treatment facilities remains an area of active research.
  • Characterizing novel bacterial species is crucial for understanding microbial ecosystems and potential applications.

Purpose of the Study:

  • To isolate and characterize a novel bacterial strain from agricultural chemical plant sludge.
  • To determine the taxonomic position of the isolated strain using polyphasic methods.
  • To propose a new species designation for the novel bacterium.

Main Methods:

  • Isolation and cultivation of bacteria from sludge samples.
  • Gram staining, motility tests, and aerobic growth assessment.
  • 16S rRNA gene sequencing for phylogenetic analysis.
  • Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) for genomic comparison.
  • Analysis of major fatty acids, predominant quinone, and polar lipid profiles.
  • Determination of genomic DNA G+C content.

Main Results:

  • Strain HX-5-24T is a Gram-negative, aerobic, non-motile rod.
  • It shares 99.1% 16S rRNA gene sequence similarity with Lysobacter dokdonensis DS-58T.
  • ANI (87.5%) and dDDH (35.3%) values indicate it is distinct from known Lysobacter species.
  • Major fatty acids include iso-C15:0, anteiso-C15:0, iso-C16:0, C16:0, and summed feature 9.
  • Genomic DNA G+C content is 66.4%.

Conclusions:

  • Strain HX-5-24T represents a novel species within the genus Lysobacter.
  • Based on phenotypic and phylogenetic data, the name Lysobacter gilvus sp. nov. is proposed.
  • The type strain is HX-5-24T (KCTC 72470T = CCTCC AB 2019228T).