ICAM-1 and MKL-1 polymorphisms impose considerable impacts on coronary heart disease occurrence

Area of Science:

• Genetics
• Cardiology
• Molecular Biology

Background:

• Coronary heart disease (CHD) is a leading cause of mortality globally.
• Genetic factors play a significant role in CHD susceptibility.
• Intercellular adhesion molecule-1 (ICAM-1) and megakaryoblastic leukaemia factor-1 (MKL-1) are implicated in inflammatory processes relevant to cardiovascular disease.

Purpose of the Study:

• To investigate the association between ICAM-1 K469E and MKL-1 -184C/T gene polymorphisms and the risk of developing CHD.
• To evaluate the role of these polymorphisms in the Chinese Han population.

Main Methods:

• A case-control study involving 100 CHD patients and 91 healthy controls.
• Genotyping of ICAM-1 and MKL-1 polymorphisms using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.
• Statistical analysis including chi-square tests and multiple logistic regression to assess correlations and adjust for traditional risk factors.

Main Results:

• The MKL-1 -184C/T polymorphism showed a statistically significant association with CHD susceptibility.
• The TT genotype frequency and T allele frequency of MKL-1 were significantly higher in the CHD patient group compared to the control group (P < .05; OR = 2.34).
• No significant association was found between the ICAM-1 K469E polymorphism and CHD risk.

Conclusions:

• The MKL-1 -184C/T polymorphism, particularly the T allele, may serve as a susceptibility factor for coronary heart disease in the Chinese Han population.
• ICAM-1 K469E polymorphism is not associated with CHD onset in this population.
• Traditional risk factors like smoking, hypertension, diabetes, and triglyceride levels remain significant contributors to CHD.