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Related Concept Videos

Urine Studies II: Urine Culture and Sensitivity Test01:26

Urine Studies II: Urine Culture and Sensitivity Test

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A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
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Asepsis01:28

Asepsis

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The condition of being free from disease-causing living pathogens is asepsis. Aseptic techniques include a set of standard practices to achieve asepsis. An example is the regular environmental cleaning of all parts of the healthcare facility and hand hygiene at home before preparing or eating food. Medical and surgical asepsis in healthcare practice protects patients from harmful pathogens, minimizes the risk of contamination of susceptible sites, and reduces the risk of infection transmission.
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Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic01:26

Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic

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Healthcare-associated infections (HAIs) occur in a healthcare facility while a person receives care for another ailment. This category also includes work-related infections among healthcare staff.
HAIs significantly increase the cost of health care. Extended stays in healthcare institutions, increased disability, increased costs of medications, including specialized antibiotics, and prolonged recovery times add to the patient's expenses and the healthcare institution and funding bodies.
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Healthcare Associated Infections II: Preventive Measures01:22

Healthcare Associated Infections II: Preventive Measures

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Essential infection prevention measures are based on the knowledge of the infection chain, the modes of transmission in healthcare settings, and the use of the best practices in all healthcare settings. Compulsory public reporting of healthcare-associated infection rates is needed to allow individuals and the community to make informed choices regarding selecting a healthcare facility.
The best practices for preventing healthcare-associated infections include hand hygiene, patient risk...
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Factors Affecting the Risk of Infection01:26

Factors Affecting the Risk of Infection

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The hosts' susceptibility to infection depends on several factors. The integrity of the skin and mucous membranes helps protect the body against microbial attacks. When the skin is altered, the chance of infection, limb loss, and even death increases.
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin...
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Sputum Studies II: Culture and Sensitivity01:20

Sputum Studies II: Culture and Sensitivity

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Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
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Related Experiment Video

Updated: Dec 12, 2025

Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
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Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression

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Culture-negative sepsis.

Jonathan Thorndike1, Marin H Kollef

  • 1Division of Pulmonary and Critical Care Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.

Current Opinion in Critical Care
|August 11, 2020
PubMed
Summary

Culture-negative sepsis treatment is challenging. Strategies like antibiotic de-escalation and rapid diagnostics may improve outcomes by reducing antibiotic use.

Area of Science:

  • Infectious Diseases
  • Critical Care Medicine
  • Clinical Microbiology

Background:

  • Traditional sepsis treatment relies on broad-spectrum antibiotics, but many patients present with culture-negative sepsis.
  • Culture-negative sepsis diagnosis and management remain challenging due to a lack of definitive microbiological data.
  • The distinction between culture-negative and culture-positive sepsis and their respective outcomes are not well-defined.

Purpose of the Study:

  • To review current understanding and recent findings regarding the management of culture-negative sepsis.
  • To explore strategies for optimizing antibiotic use in culture-negative sepsis.
  • To assess the feasibility and recommendations for antibiotic de-escalation in culture-negative sepsis.

Main Methods:

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A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
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A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis

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Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis
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Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

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Related Experiment Videos

Last Updated: Dec 12, 2025

Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
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Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression

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A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
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  • Review of recent literature and clinical recommendations on culture-negative sepsis management.
  • Analysis of outcome data comparing culture-negative and culture-positive sepsis.
  • Discussion of diagnostic challenges and therapeutic strategies, including antibiotic stewardship and rapid diagnostics.

    • Main Results:

    • Outcome data comparing culture-negative and culture-positive sepsis are mixed, with uncertainty about whether it's a distinct entity.
    • Antibiotic de-escalation is recommended and feasible in improving patients with culture-negative sepsis.
    • Strategies like limiting antibiotic duration, using rapid diagnostics, and antibiotic stewardship programs show promise.

    Conclusions:

    • Antibiotic de-escalation in culture-negative sepsis is feasible and recommended for improving patients.
    • Increased use of rapid diagnostics and antibiotic stewardship programs can lead to reduced antibiotic exposure and potentially better outcomes.
    • Further prospective data are needed to guide antibiotic choices in culture-negative sepsis due to diagnostic uncertainties.