Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Synthesis and Regulation of Thyroid Hormones

Synthesis and Regulation of Thyroid Hormones

Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...

Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...

The Thyroid Gland

The Thyroid Gland

The thyroid gland is a small, butterfly-shaped gland located in the neck and covers the anterior surface of the trachea. The gland has two lateral lobes connected by a thin tissue mass called the isthmus. Internally, each lobe comprises many small spherical structures known as thyroid follicles, surrounded by a network of blood vessels.
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Extraction, isolation, and concentration of calprotectin antigen (S100A8/S100A9) from granulocytes.

Health science reports·2019

Same author

Trans-ethnic kidney function association study reveals putative causal genes and effects on kidney-specific disease aetiologies.

Nature communications·2019

Same author

Rapid testing of red blood cells, white blood cells and platelets in intensive care patients using the HemoScreen™ point-of-care analyzer.

Platelets·2018

Same author

PhenoMeNal: processing and analysis of metabolomics data in the cloud.

GigaScience·2018

Same author

The association between circulating endostatin levels and incident myocardial infarction.

Scandinavian cardiovascular journal : SCJ·2018

Same author

Genome Analyses of >200,000 Individuals Identify 58 Loci for Chronic Inflammation and Highlight Pathways that Link Inflammation and Complex Disorders.

American journal of human genetics·2018

Search research articles

Related Experiment Video

Updated: Dec 12, 2025

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Published on: March 17, 2023

Falsely Elevated Thyroid-Stimulating Hormone Results due to Interference by M-Component of IgG-Lambda Type.

Anders Larsson¹, Mats Gåfvels¹, Torbjörn Karlsson²

¹Section of Clinical Chemistry, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.

Case Reports in Oncology

|August 11, 2020

Summary

Monoclonal immunoglobulin components (M-components) can interfere with lab tests, leading to inaccurate results. This case shows an M-component causing falsely high thyroid-stimulating hormone (TSH) levels in a myeloma patient.

Keywords:

Erroneous laboratory test result M-component Myeloma PEG- precipitation Thyroid-stimulating hormone

More Related Videos

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Author Spotlight: In Vivo Assessment of Thyroid Hormone Disruption Using the THAI Mouse Model

Author Spotlight: In Vivo Assessment of Thyroid Hormone Disruption Using the THAI Mouse Model

Published on: October 6, 2023

Related Experiment Videos

Last Updated: Dec 12, 2025

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Published on: March 17, 2023

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Author Spotlight: In Vivo Assessment of Thyroid Hormone Disruption Using the THAI Mouse Model

Author Spotlight: In Vivo Assessment of Thyroid Hormone Disruption Using the THAI Mouse Model

Published on: October 6, 2023

Area of Science:

Clinical Chemistry
Immunology
Endocrinology

Background:

Laboratory test interference can lead to misdiagnosis and inappropriate treatment.
Monoclonal immunoglobulin components (M-components), often associated with multiple myeloma, are known to cause analytical interferences.
Elevated thyroid-stimulating hormone (TSH) is a common indicator for thyroid dysfunction.

Observation:

A 75-year-old male patient with myeloma presented with persistently elevated TSH levels.
Thyroid marker analysis revealed a strong correlation between IgG concentrations and TSH values (R² = 0.724).
The highest recorded TSH level was 75 mIU/L.

Findings:

Polyethylene glycol (PEG) precipitation, used to remove intact immunoglobulins, normalized the TSH results.
This indicated that the M-component (IgG-lambda type) was causing analytical interference, not true hyperthyroidism.
The interference mechanism involved the M-component directly affecting TSH immunoassay measurements.

Implications:

Clinicians and laboratory professionals must be aware of M-component interference in immunoassays.
Consideration of M-components is crucial when interpreting abnormal thyroid function tests, especially in patients with hematological malignancies.
Implementing specific diagnostic strategies, like PEG precipitation, can help identify and circumvent M-component-related assay interferences.