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Related Experiment Video

Updated: Dec 12, 2025

Tuning the Contractility and Deformation Modes of Active Actin-Based Assemblies In Vitro: From Two-Dimensional Active Networks to Liquid Crystal Drops
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Artificial Intracellular Filaments.

Zhaoqianqi Feng1,2, Huaimin Wang1,2, Fengbin Wang3

  • 1Department of Chemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA.

Cell Reports. Physical Science
|August 11, 2020
PubMed
Summary
This summary is machine-generated.

Researchers created self-limiting peptide filaments inside living cells using enzymatic modification. These biomimetic filaments, formed from small molecules, offer insights into cellular structures and potential therapeutic applications.

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Area of Science:

  • Biomaterials Science
  • Molecular Biology
  • Cell Biology

Background:

  • Intracellular protein filaments are essential for cellular functions.
  • Creating biomimetic small-molecule filaments within living cells is challenging.

Purpose of the Study:

  • To report the in situ formation of self-limiting intracellular peptide filaments.
  • To investigate the mechanism and characteristics of these biomimetic filaments.

Main Methods:

  • Enzymatic morphological transition of a phosphorylated and trimethylated heterochiral tetrapeptide.
  • Analysis of filament structures (cross-β with C7 or C2 symmetries) and their interactions within cells.
  • Investigating the role of macromolecular crowding and peptide stereochemistry/PTMs.

Main Results:

  • Achieved in situ formation of self-limiting intracellular peptide filaments via enzymatic dephosphorylation.
  • Filaments exhibit distinct cross-β structures with hydrophilic C-terminal residues at the helix periphery.
  • Macromolecular crowding promotes bundle formation extending from plasma to nuclear membranes with minimal interaction with endogenous components.

Conclusions:

  • Peptide stereochemistry and post-translational modifications are crucial for intracellular bundle generation.
  • This approach offers a novel method for creating biomimetic intracellular structures.
  • Potential applications include restoring lost cellular functions or understanding intracellular filament-related diseases.