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Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
Published on: February 16, 2016
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Salivary proteotypes of gingivitis tolerance and resilience
Nagihan Bostanci1, Angelika Silbereisen1, Kai Bao1
1Section of Periodontology and Dental Prevention, Division of Oral Diseases, Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden.
Journal of Clinical Periodontology
|August 11, 2020
Summary
Salivary proteomic profiles reveal distinct "fast" and "slow" responders to gingivitis. These proteomic patterns, involving antigen presentation and proteolysis, may predict individual susceptibility to gum inflammation.
Area of Science:
- Oral biology
- Proteomics
- Immunology
Background:
- Gingivitis is a common inflammatory condition affecting the gums.
- Individual responses to gingivitis vary, but the underlying molecular mechanisms are not fully understood.
- Salivary proteome analysis offers a non-invasive approach to study oral health.
Purpose of the Study:
- To characterize salivary proteome changes during experimental gingivitis (EG).
- To differentiate proteomic profiles between "fast" and "slow" responders to gingivitis.
- To explore the potential of salivary proteotypes for predicting gingivitis susceptibility.
Main Methods:
- Human experimental gingivitis (EG) model induced over 21 days.
- Collection of unstimulated whole saliva at multiple time points.
- Label-free quantitative proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
- Gene Ontology (GO) enrichment analysis of regulated proteins.
Main Results:
- Quantification of 804 human proteins in saliva.
- Principal component analysis revealed significant differences between "fast" and "slow" responders.
- "Fast" responders exhibited >4-fold higher levels of 48 proteins compared to "slow" responders.
- Up-regulated proteins in "fast" responders were enriched in "antigen presentation" and "proteolysis" pathways.
Conclusions:
- Integrative systems-level quantitative proteomics can unravel "salivary proteotypes" in gingivitis.
- Distinct proteomic profiles associated with "fast" and "slow" gingivitis responses were identified.
- These differential salivary proteomic responses may aid in prognostication of individual susceptibility to gingival inflammation.
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